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不同培养基对大鼠颈上神经节组织和细胞结构的影响。

Effects of various media on tissular and cellular structures of the superior cervical ganglion of the rat.

作者信息

Pébusque M J, Robaglia A, Seïte R

出版信息

Experientia. 1979 Oct 15;35(10):1370-2. doi: 10.1007/BF01964015.

DOI:10.1007/BF01964015
PMID:499428
Abstract

The superior cervical ganglia of the rat have been incubated in vitro for 1 h in basal medium Eagle (BME) with Hanks' salts, BME with Earle's salts, Kreb's solution and NCTC 109 medium. Comparison of the cell areas, established by a semi-automatic quantitative method, shows that the three former induce a 30--35% neuronal retraction, whereas NCTC 109 has no effect. Thus this latter medium seems the best one for studies using incubation of these cells.

摘要

将大鼠的颈上神经节在含有汉克斯盐的伊格尔基础培养基(BME)、含有厄尔盐的BME、克雷布斯溶液和NCTC 109培养基中进行1小时的体外培养。通过半自动定量方法确定细胞面积后进行比较,结果表明前三种培养基会导致神经元收缩30%-35%,而NCTC 109没有影响。因此,后一种培养基似乎是用于这些细胞培养研究的最佳培养基。

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1
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Experientia. 1979 Oct 15;35(10):1370-2. doi: 10.1007/BF01964015.
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本文引用的文献

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Metabolism of glucose and oxygen in a mammalian sympathetic ganglion at reduced temperature and varied pH.低温及不同pH值条件下哺乳动物交感神经节中葡萄糖和氧气的代谢
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Effect of dibutyryl-cyclic AMP and dexamethasone on noradrenaline synthesis in isolated superior cervical ganglia.二丁酰环磷腺苷和地塞米松对离体颈上神经节去甲肾上腺素合成的影响。
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Neurons and glia in neural cultures.神经培养中的神经元和神经胶质细胞。
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