Hahn W E, Laird C D
Science. 1971 Jul 9;173(3992):158-61. doi: 10.1126/science.173.3992.158.
Under normal conditions of DNA renaturation, about 60 percent of mouse DNA fragments renature at a rate consistent with their being present only once per sperm. These nonrepeated sequences (also called single-copy or unique) may be used in RNA-DNA hybridization experiments to provide quantitative estimates of RNA diversity. About 10 percent of the mouse single-copy sequences are transcribed in mouse brain tissue. Estimates of about 3 percent were obtained for mouse liver and kidney RNA's. If only one of the complementary DNA strands is transcribed, this hybridization value implies that the equivalent of at least 300,000 different sequences of 1000 nucleotides are expressed in mouse brain tissue. It is suggested that the large amount of DNA in mammals is functionally important, and that a substantial proportion of the genome is expressed in the brain.
在DNA复性的正常条件下，约60%的小鼠DNA片段以与其在每个精子中仅出现一次相一致的速率复性。这些非重复序列（也称为单拷贝或独特序列）可用于RNA-DNA杂交实验，以提供RNA多样性的定量估计。约10%的小鼠单拷贝序列在小鼠脑组织中被转录。小鼠肝脏和肾脏RNA的估计值约为3%。如果只有互补DNA链中的一条被转录，这种杂交值意味着在小鼠脑组织中表达了至少30万个1000个核苷酸的不同序列。有人认为，哺乳动物中大量的DNA在功能上很重要，并且基因组的很大一部分在大脑中表达。
