Watson P F
Vet Rec. 1975 Jul 5;97(1):12-5. doi: 10.1136/vr.97.1.12.
Acrosomal structures of ram spermatozoa were prominently stained when air dried smears of diluted semen were fixed for 15 minutes in buffered formal saline and stained for 90 minutes in a 6 per cent (v/v) buffered solution of Giemsa stain. Progressive disruption of the acrosomes was demonstrated during chilling and deep-freezing of the spermatozoa, and the degree of damage was systematically scored. A rapid and repeatable estimate of the state of the acrosomes in a sample could be made from the mean score of 20 spermatozoa examined per slide.
当将稀释精液的空气干燥涂片在缓冲甲醛盐水中固定15分钟，并在6%（v/v）吉姆萨染色缓冲溶液中染色90分钟时，公羊精子的顶体结构被显著染色。在精子的冷藏和深度冷冻过程中，观察到顶体的渐进性破坏，并对损伤程度进行了系统评分。根据每张载玻片上检查的20个精子的平均得分，可以快速且可重复地估计样品中顶体的状态。
