Paul Dipan Rudra, Talukdar Dibyajyoti, Ahmed Fazal Ali, Lalrintluanga K, Kalita Girin, Tolenkhomba T C, Chakravarty Himsikha, Katiyar Rahul, Khargharia Gautam, Deori Sourabh
College of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, India.
Division of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, India.
Front Vet Sci. 2024 Jan 23;10:1333841. doi: 10.3389/fvets.2023.1333841. eCollection 2023.
This study was carried out to investigate the effect of different concentrations of selenium nanoparticles (Se-NPs) in the Beltsville Thawing Solution (BTS) extender on the semen quality and fertility of Hampshire crossbred pigs. For the study, semen was collected from four boars (10 ejaculates/boar) by the gloved hand method. Each ejaculate was extended @ 1:2 with the BTS extender and split into four aliquots. The control (C) samples were without the supplementation of Se-NPs, whereas the other three were supplemented with 0.5 (T1), 1 (T2), and 2 μg ml of Se-NPs (T3) and stored at 15°C in a BOD incubator. Extended semen was evaluated at 0 (immediately after dilution), 24, 48, 72, and 96 h of storage for sperm motility, live sperm, plasma membrane integrity, acrosome integrity, DNA integrity, and mitochondrial membrane potential (MMP). The mean percentage of sperm motility, live sperm, and sperm with intact plasma membrane and acrosome, and MMPs were significantly ( < 0.01) higher in all treated groups in comparison to control at 24, 48, 72, and 96 h of storage. Sperm with intact DNA in all treated groups increased significantly at 48 ( < 0.05), and 72 and 96 ( < 0.01) h of storage in comparison to the control group. The concentration of 1 μg ml of Se-NPs was found to be the best among other concentrations. In each group, 10 sows were artificially inseminated with the liquid semen preserved for 72 h at 15°C. Supplementation of 1 μg ml of Se-NPs yielded the highest conception rate in comparison to other groups. In conclusion, supplementation of 1 μg ml of Se-NPs in the BTS extender resulted in the best semen quality and conception rate during the short-time liquid preservation of boar semen.
本研究旨在探讨不同浓度的硒纳米颗粒（Se-NPs）添加到贝尔茨维尔解冻液（BTS）稀释剂中对汉普夏杂交猪精液品质和繁殖力的影响。在该研究中，通过戴手套采精法从4头公猪采集精液（每头公猪10次射精）。每次射精均用BTS稀释剂按1:2比例稀释，并分成4等份。对照组（C）样品不添加Se-NPs，而其他三组分别添加0.5（T1）、1（T2）和2μg/ml的Se-NPs（T3），并在15°C的生化培养箱中保存。在储存0（稀释后立即）、24、48、72和96小时时，对稀释后的精液进行精子活力、活精子、质膜完整性、顶体完整性、DNA完整性和线粒体膜电位（MMP）评估。在储存24、48、72和96小时时，所有处理组的精子活力、活精子、质膜和顶体完整的精子以及MMP的平均百分比均显著高于对照组（P<0.01）。与对照组相比，所有处理组中DNA完整的精子在储存48小时（P<0.05）、72小时和96小时（P<0.01）时显著增加。发现1μg/ml的Se-NPs浓度在其他浓度中效果最佳。每组中，10头母猪用在15°C保存72小时的液态精液进行人工授精。与其他组相比，添加1μg/ml的Se-NPs受孕率最高。总之，在BTS稀释剂中添加1μg/ml的Se-NPs可在公猪精液短期液态保存期间产生最佳的精液品质和受孕率。
