Hekman A, Shulman S
Clin Exp Immunol. 1971 Aug;9(2):147-60.
The antigens of guinea-pig sperm cells, of both the epididymal and ejaculated (or seminal) types, have been studied, using rabbit and guinea-pig antisera. Several antigens could be revealed by gel diffusion studies, using well-washed but non-ruptured sperm cells, indicating that intentional cell breaking is not essential for demonstrating the antigens. This release of soluble antigen was followed as a function of time and temperature, both as total protein in supernatants and in increasing strength of precipitation. With rabbit antiserum, epididymal sperm showed two antigens, that were also demonstrated in epididymal and testicular extract and in seminal sperm. These other materials revealed additional antigens with these antisera. Immunofluorescent staining was limited to the acrosomes. With guinea-pig antibodies, no precipitating antigen that was characteristic of sperm could be seen. These antisera showed immunofluorescent staining of the acrosomes. The staining could be distinguished, in terms of thermostability, from the staining produced by normal serum. No evidence was found for the occurrence of any sperm-coating antigens in the guinea-pig, especially since both antiseminal plasma and antivesicular fluid antisera failed to give immunofluorescent staining of the sperm cells.
利用兔和豚鼠抗血清，对附睾型和射精型（或精液型）豚鼠精子细胞的抗原进行了研究。通过凝胶扩散研究，使用充分洗涤但未破裂的精子细胞，可以揭示几种抗原，这表明故意破坏细胞对于证明抗原并非必不可少。随着时间和温度的变化，观察了可溶性抗原的释放情况，包括上清液中的总蛋白以及沉淀强度的增加。用兔抗血清时，附睾精子显示出两种抗原，这两种抗原也在附睾和睾丸提取物以及精液精子中得到证实。这些其他物质用这些抗血清显示出额外的抗原。免疫荧光染色仅限于顶体。用豚鼠抗体时，未观察到精子特有的沉淀抗原。这些抗血清显示出顶体的免疫荧光染色。就热稳定性而言，这种染色可以与正常血清产生的染色区分开来。未发现豚鼠存在任何精子包被抗原的证据，特别是因为抗精液浆和抗精囊液抗血清均未对精子细胞进行免疫荧光染色。
