Toullet F, Voisin G A, Nemirovsky M
Immunology. 1973 Apr;24(4):635-53.
By the indirect immunofluorescence technique three distinct guinea-pig spermatozoal autoantigens named S, P and T have been localized. Autoantigen S is present on proacrosomal granules, acrosomal granules, acrosome and head cap of spermatids and spermatozoa. Autoantigen P is present on the same formations except the head cap. Autoantigen T is also localized on acrosomes and head caps but probably on their membranes as well as on the cytoplasmic membranes of spermatozoa and spermatids. These three autoantigens are testis specific since they were not found in other organs in guinea-pigs; moreover S was detected on the acrosomal apparatus in rabbits, rats and mice. The natural antispermatozoal antibodies found in guinea-pig serum do not combine with autoantigens S and P, but the possibility that they could react with autoantigen T is considered. The cytological localization of S, P and T together with their previously described physicochemical and immunological properties allow a tentative explanation of their and immunopathological properties.
通过间接免疫荧光技术，已定位出三种不同的豚鼠精子自身抗原，分别命名为S、P和T。自身抗原S存在于精子细胞和精子的前顶体颗粒、顶体颗粒、顶体和顶帽上。自身抗原P存在于除顶帽外的相同结构上。自身抗原T也定位于顶体和顶帽，但可能也存在于它们的膜以及精子细胞和精子的细胞质膜上。这三种自身抗原是睾丸特异性的，因为在豚鼠的其他器官中未发现；此外，在兔、大鼠和小鼠的顶体装置上也检测到了S。在豚鼠血清中发现的天然抗精子抗体不与自身抗原S和P结合，但考虑到它们可能与自身抗原T发生反应的可能性。S、P和T的细胞学定位以及它们先前描述的物理化学和免疫学特性，为它们的免疫病理特性提供了一个初步解释。
