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血清对幼仓鼠肾细胞中淋巴细胞性脉络丛脑膜炎病毒产量的影响。

Effect of serum on the yield of lymphocytic choriomeningitis virus in baby hamster kidney cells.

作者信息

Stanwick T L, Kirk B E

出版信息

Appl Microbiol. 1971 Sep;22(3):289-93. doi: 10.1128/am.22.3.289-293.1971.

Abstract

The yields of the Armstrong and WE strains of lymphocytic choriomeningitis virus in baby hamster kidney (BHK) cells cultivated in either bovine, calf, fetal bovine, or horse serum were investigated. Lines of BHK cells were established in these sera. When the infected cell lines were observed by immunofluorescence, the per cent fluorescing cells for a given virus strain did not vary. However, for both strains, the extracellular virus yields per cell were significantly greater in the fetal bovine-cell line than in the other serum-cell lines.

摘要

研究了淋巴细胞性脉络丛脑膜炎病毒的阿姆斯特朗株和WE株在添加牛血清、小牛血清、胎牛血清或马血清培养的幼仓鼠肾(BHK)细胞中的产量。在这些血清中建立了BHK细胞系。当通过免疫荧光观察受感染的细胞系时,给定病毒株的荧光细胞百分比没有变化。然而,对于这两种病毒株,每细胞的细胞外病毒产量在胎牛血清培养的细胞系中显著高于其他血清培养的细胞系。

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