由二氧化碳全合成乙酸盐：中间体甲基四氢叶酸及叶酸的分离方法。
Total synthesis of acetate from CO 2 : methyltetrahydrofolate, an intermediate, and a procedure for separation of the folates.
作者信息
Parker D J, Wu T F, Wood H G
出版信息
J Bacteriol. 1971 Nov;108(2):770-6. doi: 10.1128/jb.108.2.770-776.1971.
Abstract
Whole-cell preparations of Clostridium thermoaceticum were exposed to a short pulse of (14)CO(2) under conditions in which double-labeled acetate was synthesized. Radioactive methyltetrahydrofolate monoglutamate, diglutamate, and triglutamates were isolated from extracts of the cells. The radioactivity was found to be exclusively in the five methyl position. The specific activities of the methyltetrahydrofolate derivatives were very high and were in accord with the proposal that methyltetrahydrofolates are the precursors of the methyl of acetate. A new method of separation of folates employing QAE-Sephadex chromatography and a linear gradient with triethylammonium bicarbonate is presented which completely resolves the common folate monoglutamates and, upon freeze-drying, yields salt-free preparations.
摘要
在合成双标记乙酸盐的条件下，将热醋梭菌的全细胞制剂暴露于短脉冲的（14）CO₂ 中。从细胞提取物中分离出放射性甲基四氢叶酸单谷氨酸、二谷氨酸和三谷氨酸。发现放射性仅存在于五个甲基位置。甲基四氢叶酸衍生物的比活性非常高，这与甲基四氢叶酸是乙酸盐甲基前体的提议一致。提出了一种使用QAE-葡聚糖凝胶色谱和碳酸氢三乙铵线性梯度分离叶酸的新方法，该方法能完全分离常见的叶酸单谷氨酸，冻干后可得到无盐制剂。
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本文引用的文献
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A study of carbon dioxide fixation by mass determination of the types of C13-acetate.通过对 C13 - 醋酸盐类型进行质量测定来研究二氧化碳固定作用。
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INCORPORATION OF C14 FROM CARBON DIOXIDE INTO SUGAR PHOSPHATES, CARBOXYLIC ACIDS, AND AMINO ACIDS BY CLOSTRIDIUM THERMOACETICUM.热醋梭菌将二氧化碳中的碳-14掺入糖磷酸酯、羧酸和氨基酸的过程
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The synthesis of one-carbon units from CO2 in Clostridium kluyveri.
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Folic acid metabolism.叶酸代谢
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Synchronization of converging metabolic pathways: activation of the Cystathionine gamma-synthase of Neurospora crassa by methyltetrahydrofolate.汇聚代谢途径的同步：甲基四氢叶酸对粗糙脉孢菌胱硫醚γ-合酶的激活作用
Proc Natl Acad Sci U S A. 1971 Feb;68(2):312-4. doi: 10.1073/pnas.68.2.312.
10
Total synthesis of acetate from CO2. 3. Inhibition by alkylhalides of the synthesis from CO2, methyltetrahydrofolate, and methyl-B12 by Clostridium thermoaceticum.由二氧化碳合成乙酸。3. 卤代烷对嗜热栖热菌利用二氧化碳、甲基四氢叶酸和甲基钴胺素合成乙酸的抑制作用。
Arch Biochem Biophys. 1971 Apr;143(2):471-84. doi: 10.1016/0003-9861(71)90232-3.
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