用血浆氚标记胸腺嘧啶核苷标记的动物对体外培养的小鼠肥大细胞瘤细胞进行标记。
Labeling of murine mastocytoma cells in vitro with plasma tritiated thymidine-labeled animals.
作者信息
Potten C S, Schaer J C
出版信息
J Cell Biol. 1971 Dec;51(3):862-8. doi: 10.1083/jcb.51.3.862.
40 min after injecting tritiated thymidine into an animal, 20-30% of the total plasma radioactivity is nonvolatile. This fraction decreases to about 6% 10 hr after the injection and 3% 24 hr after the injection. There appears to be material in this nonvolatile fraction that can label mastocytoma cells in culture. The labeling indices decrease with time after injection in the same way as the nonvolatile fraction. The 40 min plasma sample contains sufficient material to allow accurate assessment of the fraction of cells in S in culture after a 6 wk exposure. The circulating material is not apparently available for incorporation into those cells in cycle in the donor animal. The material appears to be related to the G(0) cell-specific pool that has been described elsewhere. The trichloroacetic acid-soluble or ethanol-soluble nonvolatile activity appears to contain thymine, and some thymidine-phosphorylated compounds.
向动物注射氚标记胸腺嘧啶核苷40分钟后，血浆总放射性的20 - 30%是非挥发性的。该部分在注射后10小时降至约6%，注射后24小时降至3%。在这种非挥发性部分中似乎存在能够标记培养中的肥大细胞瘤细胞的物质。标记指数在注射后随时间下降，方式与非挥发性部分相同。40分钟的血浆样本含有足够的物质，可在6周暴露后准确评估培养中处于S期的细胞比例。循环中的物质显然无法用于供体动物中处于细胞周期的那些细胞的掺入。该物质似乎与其他地方描述的G(0)细胞特异性池有关。三氯乙酸可溶性或乙醇可溶性非挥发性活性似乎含有胸腺嘧啶以及一些胸腺嘧啶磷酸化化合物。
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