Higashino K, Otani R, Kudo S, Hashinostume M, Hada T
Ann Intern Med. 1975 Jul;83(1):74-8. doi: 10.7326/0003-4819-83-1-74.
Four patients with hepatocellular carcinoma had a variant alkaline phosphatase that resembles the placental D-variant but is different from it in electrophoretic mobility, pH optimum, heat stability, and inhibition by phosphate. The appearance of this enzyme has been specific to hepatocellular carcinoma. Its prevalence was about 30%, while that of another marker protein, alpha-fetoprotein was 77%. The occurrence of this enzyme in serum of patients with hepatoma was, accordingly, independent of the serum alpha-fetoprotein concentration, and also independent of the appearance of the Regan or the Nagao isoenzymes and of the serum alkaline phosphatase activity. Patients with the enzyme had a massive type of hepatocellular carcinoma with grade III differentiation by Edmondson's classification. The detection of this enzyme in serum may be of help in confirming the diagnosis of hepatoma.
四名肝细胞癌患者有一种变异碱性磷酸酶，它类似于胎盘D变异体，但在电泳迁移率、最适pH值、热稳定性和对磷酸盐的抑制作用方面与之不同。这种酶的出现对肝细胞癌具有特异性。其发生率约为30%，而另一种标志物蛋白甲胎蛋白的发生率为77%。因此，这种酶在肝癌患者血清中的出现与血清甲胎蛋白浓度无关，也与Regan或Nagao同工酶的出现以及血清碱性磷酸酶活性无关。患有这种酶的患者患有Edmondson分类法中III级分化的大块型肝细胞癌。血清中这种酶的检测可能有助于确诊肝癌。
