• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于纯化酸提取的A组链球菌M蛋白的方法评估

Evaluation of methods used to purify acid-extracted group A streptococcal M protein.

作者信息

Phillips L S, Pine L

出版信息

Appl Microbiol. 1971 Dec;22(6):963-73. doi: 10.1128/am.22.6.963-973.1971.

DOI:10.1128/am.22.6.963-973.1971
PMID:5002902
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376470/
Abstract

The literature includes descriptions of both acid-soluble and acid-insoluble M protein in the preparation of "hot acid-extracted group A streptococcal M protein." We present evidence for the contamination of crude type 1 acid-insoluble M protein. The purification of preparations of crude and partially purified acid-soluble type 1 and type 12 M protein is described. Our quantitative criteria for purification were recovery of M precipitin activity, improvement in specific activity, and removal of carbohydrate. Exclusion of nucleic acid is also discussed. Greater purification in a single passage was found with a carboxymethylcellulose column (with acidic elution) than with hydroxyapatite, diethylaminoethyl-Sephadex, or carboxymethyl-cellulose (with neutral elution) columns or with ammonium sulfate fractional precipitation. Carboxymethylcellulose with acidic elution was found to be a satisfactory standard laboratory procedure for the preparation of purified acid-extracted (acid-soluble) group A streptococcal M protein.

摘要

文献中记载了在制备“热酸提取的A组链球菌M蛋白”时酸溶性和酸不溶性M蛋白的情况。我们提供了粗制1型酸不溶性M蛋白受到污染的证据。本文描述了粗制和部分纯化的酸溶性1型和12型M蛋白制剂的纯化过程。我们的纯化定量标准是M沉淀素活性的恢复、比活性的提高以及碳水化合物的去除。还讨论了核酸的排除。结果发现，与羟基磷灰石、二乙氨基乙基葡聚糖、羧甲基纤维素（中性洗脱）柱或硫酸铵分级沉淀相比，使用羧甲基纤维素柱（酸性洗脱）单次纯化的效果更好。酸性洗脱的羧甲基纤维素被认为是制备纯化的酸提取（酸溶性）A组链球菌M蛋白的一种令人满意的标准实验室方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/691f38c6b90b/applmicro00122-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/b2eb9ffe7945/applmicro00122-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/d9c90826b539/applmicro00122-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/65b8d0eef1d1/applmicro00122-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/691f38c6b90b/applmicro00122-0047-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/b2eb9ffe7945/applmicro00122-0046-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/d9c90826b539/applmicro00122-0046-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/65b8d0eef1d1/applmicro00122-0046-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0f00/376470/691f38c6b90b/applmicro00122-0047-a.jpg

相似文献

1
Evaluation of methods used to purify acid-extracted group A streptococcal M protein.用于纯化酸提取的A组链球菌M蛋白的方法评估
Appl Microbiol. 1971 Dec;22(6):963-73. doi: 10.1128/am.22.6.963-973.1971.
2
Simplified method for the purification of group A streptococcal M-proteins: solution of the multiple banding problem.A组链球菌M蛋白纯化的简化方法：多带问题的解决方案
Appl Microbiol. 1974 Jan;27(1):28-37. doi: 10.1128/am.27.1.28-37.1974.
3
Immunological characteristics of heat-killed Streptococcus type 1--sonic fractions obtained by zonal fractionation.1型热灭活链球菌的免疫学特性——通过区带分级分离获得的超声破碎组分
Microbios. 1971 Apr;3(12):225-46.
4
Chemistry and end-group analysis on purified M protein of type 12 group A streptococcal cell walls.A群12型链球菌细胞壁纯化M蛋白的化学与端基分析
J Bacteriol. 1969 Dec;100(3):1277-83. doi: 10.1128/jb.100.3.1277-1283.1969.
5
Guanidine extraction of streptococcal M protein.链球菌M蛋白的胍提取法。
Infect Immun. 1975 Sep;12(3):679-86. doi: 10.1128/iai.12.3.679-686.1975.
6
Characterization of group A streptococcal R-28 antigen purified by hydroxyapatite column chromatography.通过羟基磷灰石柱色谱法纯化的A组链球菌R-28抗原的特性分析。
Infect Immun. 1975 Oct;12(4):901-9. doi: 10.1128/iai.12.4.901-909.1975.
7
The antigenic heterogenity of M protein of Streptococcus pyogenes (type 24).化脓性链球菌（24型）M蛋白的抗原异质性
Zentralbl Bakteriol Orig. 1970;214(3):390-2.
8
The characteristics of M proteins purified by column chromatography with hydroxyapatite from acid extracts of Streptococcus pyogenes of types 1, 3, 6, 12 and 17.用羟基磷灰石柱色谱法从1、3、6、12和17型化脓性链球菌的酸提取物中纯化的M蛋白的特性。
J Med Microbiol. 1978 Nov;11(4):453-62. doi: 10.1099/00222615-11-4-453.
9
Comparison of Streptomyces albus muramidase-extracted streptococcal antigen with acid-extracted M antigen and with pepsin-extracted T antigen.白色链霉菌溶菌酶提取的链球菌抗原与酸提取的M抗原及胃蛋白酶提取的T抗原的比较。
Infect Immun. 1977 Apr;16(1):310-7. doi: 10.1128/iai.16.1.310-317.1977.
10
Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.通过离子交换柱色谱法纯化的A组链球菌T-12蛋白的特性分析。
Infect Immun. 1978 Sep;21(3):940-5. doi: 10.1128/iai.21.3.940-945.1978.

引用本文的文献

1
Purification, composition, and serological characterization of histoplasmin-H and M antigens.组织胞浆菌素-H和-M抗原的纯化、组成及血清学特性
Infect Immun. 1974 May;9(5):870-80. doi: 10.1128/iai.9.5.870-880.1974.
2
M proteins of group A streptococci.A组链球菌的M蛋白
Bacteriol Rev. 1974 Mar;38(1):57-86. doi: 10.1128/br.38.1.57-86.1974.
3
The cell surface antigens of Streptococcus equi.马链球菌的细胞表面抗原

本文引用的文献

1
Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus.从A群1型溶血性链球菌中分离出的型特异性M抗原的制备及特性
J Exp Med. 1952 Jul;96(1):71-82. doi: 10.1084/jem.96.1.71.
2
Extraction of the T antigen of Streptococcus pyogenes.化脓性链球菌T抗原的提取。
J Gen Microbiol. 1951 Oct;5(4):640-7. doi: 10.1099/00221287-5-4-640.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
Can J Comp Med. 1975 Apr;39(2):110-5.
4
Biochemical and serological characteristics of soluble yeast phase antigens of Histoplasma capsulatum.荚膜组织胞浆菌可溶性酵母相抗原的生化及血清学特征
Mycopathologia. 1979 Mar 30;67(1):3-16. doi: 10.1007/BF00436234.
J Biol Chem. 1951 Nov;193(1):265-75.
4
EPIDEMIOLOGIC CHARACTERIZATION OF GROUP A STREPTOCOCCI BY T-AGGLUTINATION AND M-PRECIPITATION TESTS IN THE PUBLIC HEALTH LABORATORY.公共卫生实验室中通过T凝集试验和M沉淀试验对A群链球菌进行的流行病学特征分析
Health Lab Sci. 1965 Jul;2:149-62.
5
ANTIGENICITY OF THE M PROTEINS OF GROUP A HEMOLYTIC STREPTOCOCCI.A 群溶血性链球菌 M 蛋白的抗原性
J Immunol. 1964 Nov;93:826-37.
6
[NEW METHOD OF EXTRACTION AND PURIFICATION OF THE M PROTEIN OF STREPTOCOCCUS PYOGENES OF THE A GROUP APPLIED TO TYPE 24].[应用于24型的A组化脓性链球菌M蛋白提取与纯化的新方法]
Ann Inst Pasteur (Paris). 1963 Dec;105:1063-70.
7
THE CELLULAR ANTIGENS OF GROUP A STREPTOCOCCI; IMMUNOELECTROPHORETIC STUDIES OF THE C, M, T, PGP, E4, F, AND E ANTIGENS OF SEROTYPE 17 STREPTOCOCCI.A 群链球菌的细胞抗原；17 型链球菌 C、M、T、PGP、E4、F 和 E 抗原的免疫电泳研究
J Exp Med. 1963 Oct 1;118(4):527-56. doi: 10.1084/jem.118.4.527.
8
EFFECT OF ANTIBIOTICS AND INHIBITORS ON M PROTEIN SYNTHESIS.抗生素和抑制剂对M蛋白合成的影响。
J Bacteriol. 1963 Mar;85(3):527-31. doi: 10.1128/jb.85.3.527-531.1963.
9
Enzyme fractionation by salting-out: a theoretical note.通过盐析进行酶分级分离：一篇理论笔记。
Adv Protein Chem. 1961;16:197-219. doi: 10.1016/s0065-3233(08)60030-3.
10
The occurrence of polyglycerophosphate as an antigenic component of various gram-positive bacterial species.聚甘油磷酸作为多种革兰氏阳性菌的一种抗原成分的出现情况。
J Exp Med. 1959 Apr 1;109(4):361-78. doi: 10.1084/jem.109.4.361.