Collste L G, Kostyrka-Claps M L, Darzynkiewicz Z, Devonec M, Traganos F, Whitmore W F, Melamed M R
Invest Urol. 1979 Nov;17(3):191-4.
Lymph node lymphocyte reaction to an explanted, transplantable mouse bladder tumor (MBT-2) was investigated by flow cytometry in animals previously immunized with irradiated tumor cells. Nodal lymphocytes in representative samples from four different lymph node sites were differentially stained for DNA and RNA with the fluorescent dye acridine orange; cell proliferation and the increase in RNA content were measured. Immunization abrogated tumor growth; one immunization reduced tumor take to 25 per cent of the animals, and two immunizations to 14 per cent. Lymphocyte reactivity to the tumor was reflected both by an increase of DNA synthesizing cells and by diploid cells with high RNA. The latter response was more pronounced and thus the more sensitive parameter for measuring immunologic lymph node reactivity. The juxtatumoral node displayed the most pronounced reactivity, but all node sites showed some degree of reaction.
通过流式细胞术研究了在先前用辐照肿瘤细胞免疫的动物中，淋巴结淋巴细胞对移植性小鼠膀胱肿瘤（MBT-2）的反应。用荧光染料吖啶橙对来自四个不同淋巴结部位的代表性样本中的淋巴结淋巴细胞进行DNA和RNA差异染色；测量细胞增殖和RNA含量的增加。免疫可抑制肿瘤生长；一次免疫使肿瘤接种率降低至动物总数的25%，两次免疫则降至14%。淋巴细胞对肿瘤的反应既表现为DNA合成细胞的增加，也表现为RNA含量高的二倍体细胞的增加。后一种反应更为明显，因此是测量免疫淋巴结反应性的更敏感参数。肿瘤旁淋巴结显示出最明显的反应，但所有淋巴结部位均表现出一定程度的反应。
