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作为对经肠外免疫小鼠引流淋巴结中细胞介导免疫的体外检测方法的淋巴结淋巴细胞迁移抑制

Migration inhibition of lymph node lymphocytes as an in vitro assay for cell-mediated immunity in the draining lymph nodes of parenterally immunized mice.

作者信息

Mowat A M, Ferguson A

出版信息

Immunology. 1982 Oct;47(2):357-64.

PMID:6180978
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1555464/
Abstract

This paper describes a method for in vitro measurement of specific cell-mediated immunity in the mouse. Animals were immunized parenterally with ovalbumin in Freund's incomplete or complete adjuvant, and a direct migration inhibition assay was performed, using lymphoid cells from the draining lymph nodes. Migration inhibition was found to be antigen specific, correlated with systemic delayed-type hypersensitivity measured in vivo by skin testing, and had a high degree of sensitivity for ovalbumin. The migrating cells were identified as lymphocytes. Lymph node lymphocyte migration inhibition provides a reliable in vitro assay for regional CMI in the mouse.

摘要

本文描述了一种在小鼠中体外测量特异性细胞介导免疫的方法。用弗氏不完全或完全佐剂中的卵清蛋白对动物进行非肠道免疫，然后使用引流淋巴结中的淋巴细胞进行直接迁移抑制试验。发现迁移抑制具有抗原特异性，与通过皮肤试验在体内测量的全身性迟发型超敏反应相关，并且对卵清蛋白具有高度敏感性。迁移细胞被鉴定为淋巴细胞。淋巴结淋巴细胞迁移抑制为小鼠的局部细胞介导免疫提供了一种可靠的体外检测方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a4/1555464/fa5f9d263348/immunology00223-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a4/1555464/fa5f9d263348/immunology00223-0138-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/64a4/1555464/fa5f9d263348/immunology00223-0138-a.jpg

相似文献

1
Migration inhibition of lymph node lymphocytes as an in vitro assay for cell-mediated immunity in the draining lymph nodes of parenterally immunized mice.作为对经肠外免疫小鼠引流淋巴结中细胞介导免疫的体外检测方法的淋巴结淋巴细胞迁移抑制
Immunology. 1982 Oct;47(2):357-64.
2
Migration inhibition of lymph node lymphocytes as an assay for regional cell-mediated immunity in the intestinal lymphoid tissues of mice immunized orally with ovalbumin.以淋巴结淋巴细胞迁移抑制作为检测用卵清蛋白经口免疫的小鼠肠道淋巴组织中局部细胞介导免疫的方法。
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[Specific inhibition of lymphocytes, proliferating in response to an antigen, in mouse lymph nodes containing the same antigen].[在含有相同抗原的小鼠淋巴结中，对因抗原而增殖的淋巴细胞的特异性抑制作用]
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引用本文的文献

1
Immunological responses to fed protein antigens in mice. IV. Effects of stimulating the reticuloendothelial system on oral tolerance and intestinal immunity to ovalbumin.小鼠对摄入蛋白质抗原的免疫反应。IV. 刺激网状内皮系统对口服耐受及对卵清蛋白的肠道免疫的影响。
Immunology. 1983 Dec;50(4):547-54.
2
Establishment of an animal model of ovalbumin sensitised mouse to study protein induced enteropathy.建立卵清蛋白致敏小鼠动物模型以研究蛋白质诱导的肠病。
Gut. 1986 Nov;27(11):1298-305. doi: 10.1136/gut.27.11.1298.
3
Depletion of suppressor T cells by 2'-deoxyguanosine abrogates tolerance in mice fed ovalbumin and permits the induction of intestinal delayed-type hypersensitivity.

本文引用的文献

1
Hypersensitivity in the small intestinal mucosa. V. Induction of cell-mediated immunity to a dietary antigen.小肠黏膜中的超敏反应。V. 对饮食抗原的细胞介导免疫的诱导。
Clin Exp Immunol. 1981 Mar;43(3):574-82.
2
Lymphocyte locomotion. The role of chemokinesis and chemotaxis.淋巴细胞运动。化学增活作用和趋化性的作用。
Monogr Allergy. 1980;16:173-86.
3
Hypersensitivity reactions in the small intestine. 6. Pathogenesis of the graft-versus-host reaction in the small intestinal mucosa of the mouse.小肠中的超敏反应。6. 小鼠小肠黏膜移植物抗宿主反应的发病机制。
用2'-脱氧鸟苷耗尽抑制性T细胞可消除喂食卵清蛋白的小鼠的耐受性，并允许诱导肠道迟发型超敏反应。
Immunology. 1986 Jun;58(2):179-84.
Transplantation. 1981 Sep;32(3):238-43. doi: 10.1097/00007890-198109000-00011.
4
The differential localization of antibody synthesis and of immunologic memory in lymph nodes draining and not draining the site of primary immunization with hemocyanin.在用血蓝蛋白进行初次免疫的部位引流和不引流的淋巴结中，抗体合成和免疫记忆的差异定位。
J Immunol. 1972 Jan;108(1):152-60.
5
In vitro approaches to the mechanism of cell-mediated immune reactions.细胞介导免疫反应机制的体外研究方法。
Adv Immunol. 1971;13:101-208. doi: 10.1016/s0065-2776(08)60184-4.
6
Cells involved in cell-mediated and transplantation immunity in the rabbit. IV. The organ localization of the cells capable of migrating in vitro. Inhibition of migration by immunizing antigen.家兔中参与细胞介导免疫和移植免疫的细胞。IV. 体外可迁移细胞的器官定位。免疫抗原对迁移的抑制作用。
Cell Immunol. 1972 Nov;5(3):377-91. doi: 10.1016/0008-8749(72)90064-0.
7
A rapid method for the isolation of functional thymus-derived murine lymphocytes.一种分离功能性胸腺来源的小鼠淋巴细胞的快速方法。
Eur J Immunol. 1973 Oct;3(10):645-9. doi: 10.1002/eji.1830031011.
8
Migration enhancement factor: a new lymphokine.迁移增强因子：一种新的淋巴因子。
Proc Natl Acad Sci U S A. 1974 Mar;71(3):875-9. doi: 10.1073/pnas.71.3.875.
9
Antigen-induced inhibition of cell migration in agar gel, plasma clot, and liquid media.抗原诱导的琼脂凝胶、血浆凝块和液体培养基中细胞迁移的抑制。
J Reticuloendothel Soc. 1968 Oct;5(5):472-83.
10
Lymphokines in sensitized rats. I. Migration inhibitory factor(s) from specifically stimulated thymocytes in vitro.致敏大鼠中的淋巴细胞因子。I. 体外特异性刺激胸腺细胞产生的迁移抑制因子
Int Arch Allergy Appl Immunol. 1975;49(4):491-503.