Saborio J L, Segura M, Flores M, Garcia R, Palmer E
J Biol Chem. 1979 Nov 10;254(21):11119-25.
The iso forms of gizzard actin present during chicken embryogenesis were analyzed by two-dimensional electrophoresis. During chick embryogenesis there are conspicuous changes in the content of the iso forms of actin. Gizzards from 8-day-old embryos contain almost exclusively beta-actin. After 8 days of embryonic age, there is a continuous increase in the amount of gamma-actin and an apparent decrease in the amount of beta-actin. These changes in the content of beta- and gamma-actin in gizzard tissue are paralleled by changes in the content of the corresponding mRNAs, as detected by the ability of the RNAs to direct the synthesis of these proteins in micrococcal nuclease-digested reticulocyte lysates. The correlation of the in vivo and in vitro experiments indicates that during chick embryogenesis there is a differential expression of the genes coding for the iso forms of gizzard actin and that this expression is controlled at the transcriptional level.
通过二维电泳分析了鸡胚胎发育过程中砂囊肌动蛋白的同工型。在鸡胚胎发育过程中，肌动蛋白同工型的含量有明显变化。8日龄胚胎的砂囊中几乎只含有β-肌动蛋白。胚胎发育8天后，γ-肌动蛋白的量持续增加，而β-肌动蛋白的量明显减少。砂囊组织中β-肌动蛋白和γ-肌动蛋白含量的这些变化与相应mRNA含量的变化平行，这是通过RNA在微球菌核酸酶消化的网织红细胞裂解物中指导这些蛋白质合成的能力检测到的。体内和体外实验的相关性表明，在鸡胚胎发育过程中，编码砂囊肌动蛋白同工型的基因存在差异表达，并且这种表达在转录水平上受到控制。
