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储存条件对人血清氨基酸浓度的影响(作者译)

[Influence of storing conditions on the amino acid concentration in human serum (author's transl)].

作者信息

Olek K, Uhlhaas S, Wardenbach P, Yamaguchi M

出版信息

J Clin Chem Clin Biochem. 1979 Sep;17(9):599-604.

PMID:501307
Abstract

Whole blood, serum and deproteinized serum were stored at room temperature up to 24 h and at - 30 degrees C up to one month. The amino acid content was then determined with an automatic amino acid analyser. When whole blood is left at room temperature the concentration of citrulline, alpha-aminobutyric acid, cysteine and tryptophan remains unchanged, whereas the other amino acids show a remarkable increase or decrease. In serum stored for 24 h at room temperature, only the concentrations of aspartic and glutamic acid, serine, cysteine and phenylalanine were altered. With the exception of aspartic and glutamic acid it was possible to leave deproteinized serum up to 24 h at room temperature and up to one month at - 30 degrees C without observing a change in the concentration of the other amino acids. No change occurred, when serum was stored at - 30 degrees C for 24 h.

摘要

全血、血清和脱蛋白血清在室温下可保存24小时,在-30℃下可保存一个月。然后用自动氨基酸分析仪测定氨基酸含量。当全血在室温下放置时,瓜氨酸、α-氨基丁酸、半胱氨酸和色氨酸的浓度保持不变,而其他氨基酸则有显著增加或减少。血清在室温下保存24小时,只有天冬氨酸、谷氨酸、丝氨酸、半胱氨酸和苯丙氨酸的浓度发生改变。除天冬氨酸和谷氨酸外,脱蛋白血清在室温下可保存24小时,在-30℃下可保存一个月,而其他氨基酸的浓度没有变化。血清在-30℃下保存24小时,没有变化。

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