Hopper A F, Rose P M, Wannemacher R W
J Cell Biol. 1972 Apr;53(1):225-30. doi: 10.1083/jcb.53.1.225.
The effect of protein-free and starvation diets on the migration of cells from the crypts onto and up the villi of the rat ileum was studied. Rats starved for 3, 7, or 10 days or fed a protein-free diet (PFD) for 3, 7, or 11 wk were injected with thymidine-(3)H and sacrificed at timed intervals. The time required for the labeled cells to first appear on the villi of experimental animals was longer than in the controls. This was the result of an elongated cycle in the protein-depleted animals and a lengthening of the maturation period in both the starved and protein-depleted animals. Determination of the distance which labeled cells had migrated up the villi in control and experimental animals, after thymidine-(3)H injection, indicated that cells in animals starved for 7 days migrated more rapidly than those in the fed controls, while those of 10-day starved animals moved more slowly. The cells of animals fed PFD for 3 wk migrated up the villi more rapidly, those of animals depleted for 7 wk migrated at the same time rate, and those of 11-wk PFD animals migrated more slowly than the fed controls. There is apparently no correlation between the cell cycle time in the crypt cells and the rate of migration of cells up the villus.
研究了无蛋白饮食和饥饿饮食对大鼠回肠隐窝细胞向绒毛迁移及沿绒毛向上迁移的影响。对饥饿3天、7天或10天或喂食无蛋白饮食（PFD）3周、7周或11周的大鼠注射氚标记胸腺嘧啶核苷，并在不同时间间隔处牺牲。标记细胞首次出现在实验动物绒毛上所需的时间比对照组更长。这是蛋白质缺乏动物细胞周期延长以及饥饿和蛋白质缺乏动物成熟时间延长的结果。在注射氚标记胸腺嘧啶核苷后，测定对照和实验动物中标记细胞沿绒毛向上迁移的距离，结果表明，饥饿7天的动物中的细胞比喂食的对照动物中的细胞迁移得更快，而饥饿10天的动物中的细胞迁移得更慢。喂食PFD 3周的动物的细胞沿绒毛向上迁移得更快，缺乏7周的动物的细胞迁移速度相同，而喂食PFD 11周的动物的细胞迁移得比喂食的对照动物更慢。隐窝细胞的细胞周期时间与细胞沿绒毛向上迁移的速度之间显然没有相关性。
