饥饿对大鼠肝脏和小肠蛋白质合成速率的影响。
The effect of starvation on the rate of protein synthesis in rat liver and small intestine.
作者信息
McNurlan M A, Tomkins A M, Garlick P J
出版信息
Biochem J. 1979 Feb 15;178(2):373-9. doi: 10.1042/bj1780373.
Abstract
- A method is described that allows for measurement of protein synthesis in liver and intestine in the rat. By injecting a massive amount of [14C]leucine (100 mumol/100 g body wt.) an attempt has been made to over come problems of precursor specific radioactivity and problems arising from the breakdown of labelled protein that are encountered when tracer amounts of amino acids are used. 2. Starvation for 2 days resulted in decline in the rate of total liver protein synthesis from 87%/day to 62%/day. 3. In jejunal mucosa the rate of protein synthesis was 136%/day. This declined to 105%/day after 2 days of starvation.
摘要
- 本文描述了一种可用于测量大鼠肝脏和肠道中蛋白质合成的方法。通过注射大量的[14C]亮氨酸（100微摩尔/100克体重），试图克服使用微量氨基酸作为示踪剂时遇到的前体比放射性问题以及标记蛋白质分解所产生的问题。2. 饥饿2天导致肝脏总蛋白质合成速率从每天87%降至每天62%。3. 在空肠黏膜中，蛋白质合成速率为每天136%。饥饿2天后，该速率降至每天105%。
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