Sanders J H
Clin Exp Immunol. 1972 Jan;10(1):191-7.
A microdroplet technique for assaying antibody using rat peritoneal macrophage monolayers is presented. Its reliability is assessed with duplicate and repetitive performance using samples of normal and anti-lymphocytic equine globulin. The specificity of the test for is indicated in a second series of tests by the use of globulin fractions with widely varing cytotoxic, lymph agglutinating and immunosuppressive activities.
本文介绍了一种使用大鼠腹膜巨噬细胞单层检测抗体的微滴技术。通过使用正常样本和抗淋巴细胞马球蛋白样本进行重复检测来评估其可靠性。在第二系列测试中,通过使用具有广泛不同细胞毒性、淋巴细胞凝集和免疫抑制活性的球蛋白组分来表明该测试的特异性。