The mediating effect of anti-rat lymphocytic globulin on the uptake of homologous lymphocytes by rat macrophages.

The uptake of homologous lymphocytes by rat macrophages was observed in tissue culture chambers. This uptake was quantified as an opsonization index. The effect on this index of treatment of either lymphocytes or macrophages by horse anti-lymphocytic globulin (ALG) or normal horse IgG (N.IgG) in the presence or absence of complement was measured. ALG-treated lymphocytes were shown to attach to immune adherence receptors on human erythrocytes in the presence of complement. A complement attachment of ALG-treated lymphocytes to macrophages was also observed following blocking of cytophilic receptors by N.IgG.