犬肾素中分子量转化与肾素活性之间的关系。

Relationship between molecular weight conversion and renin activity in dog renal renin.

作者信息

Funakawa S, Ikemoto F, Kawamura M, Yamamoto K

出版信息

Jpn Circ J. 1979 Sep;43(9):824-6. doi: 10.1253/jcj.43.824.

DOI:10.1253/jcj.43.824

PMID:501902

Abstract

A normal size form of renin, which seems to be a storage form in renin granules, changed neither in molecular weight nor activity by acidification to pH 3.0. High molecular weight (HMW) renin fractionated by gel chromatography from crude renal extract prepared with thiol group blockers was converted into normal size renin by acdification, accompanied with an increase in renin activity by about 50%. The molecular weight conversion by acidification appeared due to destruction or loss of binding ability of the renin binding substance which was present in the cytosol of renal cortical tissue. Renin and renin binding substance could combine into HMW renin at neutral pH in the presence of thiol group blockers and renin activity decreased.

摘要

正常大小的肾素形式，似乎是肾素颗粒中的一种储存形式，通过酸化至pH 3.0，其分子量和活性均未改变。用硫醇基团阻断剂制备的粗肾提取物经凝胶色谱分离得到的高分子量（HMW）肾素，通过酸化转化为正常大小的肾素，同时肾素活性增加约50%。酸化引起的分子量转化似乎是由于肾皮质组织细胞质中存在的肾素结合物质的结合能力被破坏或丧失所致。在硫醇基团阻断剂存在的情况下，肾素和肾素结合物质在中性pH下可结合形成HMW肾素，且肾素活性降低。