Ikemoto F, Kawamura M, Takaori K, Yamamoto K
Jpn Circ J. 1980 May;44(5):371-4. doi: 10.1253/jcj.44.371.
Our recent findings on molecular weight conversion in the case of renin are summarized and reviewed herein. The molecular weight of renin extracted from isolated renin granules from dogs and rats is approximately 40,000. This renin reacts with a constituent of kidney extract to form a high molecular weight renin of approximately 60,000. This constituent was termed a renin binding substance. This substance is probably contained in the cytosol of kidney cortex, and its characteristics are (1) heat instability, (2) non-dialyzability, (3) loss of binding ability by acidification at pH 3.0 and (4) apparent molecular weight is over 47,000. The binding reaction is mediated in two different ways; thiol oxidation with sodium tetrathionate and exposure of the reaction mixture at 0 degrees C for several days without thiol oxidation.
本文总结并回顾了我们最近关于肾素分子量转换的研究结果。从狗和大鼠的分离肾素颗粒中提取的肾素分子量约为40,000。这种肾素与肾提取物的一种成分反应，形成分子量约为60,000的高分子量肾素。这种成分被称为肾素结合物质。该物质可能存在于肾皮质的胞质溶胶中，其特征为：（1）热不稳定性；（2）不可透析性；（3）在pH 3.0时酸化会导致结合能力丧失；（4）表观分子量超过47,000。结合反应通过两种不同方式介导：用连四硫酸钠进行硫醇氧化，以及在无硫醇氧化的情况下将反应混合物在0℃下放置数天。
