Funakawa S, Funae Y, Yamamoto K
Biochem J. 1978 Dec 15;176(3):977-81. doi: 10.1042/bj1760977.
Two forms of renin, one of mol.wt. 43,000 and the other 60,000, were found in the dog kidney. Conversion between the two forms of renin was reversible at neutral pH. Though the molecular weight of renin in kidney-cortex homogenate was 43,000, it was completely converted into high-molecular-weight renin in the presence of substances that react with thiol groups. On the contrary, stored renin in the granules was the form of normal size (mol. wt. 43,000) regardless of the absence or presence of such substances. The present experiments indicated that renin is stored in the granules as the form of normal size and might be converted into high-molecular-weight renin when it is released from the granules and attached to some substance in the soluble fraction of renal-cortical tissue.
在狗肾中发现了两种形式的肾素，一种分子量为43,000，另一种为60,000。两种形式的肾素在中性pH条件下的转化是可逆的。尽管肾皮质匀浆中肾素的分子量为43,000，但在存在与巯基反应的物质时，它会完全转化为高分子量肾素。相反，颗粒中储存的肾素无论是否存在此类物质，均为正常大小的形式（分子量43,000）。目前的实验表明，肾素以正常大小的形式储存在颗粒中，当它从颗粒中释放并附着于肾皮质组织可溶性部分中的某些物质时，可能会转化为高分子量肾素。
