Malley A, Baecher L, Begley D
Dev Biol Stand. 1975;29:29-40.
Previous studies with timothy pollen extracts demonstrated that a low molecular weight dialyzable fraction, antigen D, possessed the allergenic determinant of the major allergen of timothy pollen (allergen B). Bio-gel P-2 gel-filtration of the dialysate fraction resulted in the isolation of a fragment, antigen D3 with a molecular weight near 1000. The relationship of antigen D3 to the antigenic and allergenic determinants of allergen B was evaluated by passive cutaneous anaphylaxis (PCA) in guinea pigs sensitized with rabbit antitimothy sera and by allergen-induced histamine release from monkey lung tissue passively sensitized with the serum of timothy-sensitive patients. These studies demonstrated that 10 units of antigen D3 gave 1) a 70% inhibition of PCA reactions in guinea pigs challenged with allergen B, and 2) a 73% inhibition of allergen-induced histamine release from monkey lung tissue sensitized with timothy reagin and challenged with the crude pollen extract (WST). Although chemical characterization studies of the antigen D3 fragment are still being carried out it appears that the major components fo this fragment are 1) a flavonoid pigment - quercitin, 2) a disaccharide moiety - cellobiose, and 3) the amino acid threonine linked together in an )O-glycosidic type linkage.
先前对梯牧草花粉提取物的研究表明，一种低分子量的可透析组分，即抗原D，具有梯牧草花粉主要过敏原（过敏原B）的致敏决定簇。对透析液组分进行生物凝胶P-2凝胶过滤，分离出了一个分子量接近1000的片段，即抗原D3。通过被动皮肤过敏反应（PCA）在经兔抗梯牧草血清致敏的豚鼠中以及通过过敏原诱导的组胺从经梯牧草敏感患者血清被动致敏的猴肺组织中释放，评估了抗原D3与过敏原B的抗原决定簇和致敏决定簇之间的关系。这些研究表明，10个单位的抗原D3可：1）使用过敏原B攻击的豚鼠的PCA反应受到70%的抑制，以及2）使经梯牧草反应素致敏并用粗花粉提取物（WST）攻击的猴肺组织中过敏原诱导的组胺释放受到73%的抑制。尽管对抗原D3片段的化学特性研究仍在进行，但该片段的主要成分似乎是：1）一种类黄酮色素——槲皮素，2）一个二糖部分——纤维二糖，以及3）通过O-糖苷型连接连接在一起的氨基酸苏氨酸。
