γ-羟基丁酸对大鼠纹状体单胺释放的影响。
Effect of -hydroxybutyrate on the release of monoamines from the rat striatum.
作者信息
Bustos G, Roth R H
出版信息
Br J Pharmacol. 1972 Apr;44(4):817-20. doi: 10.1111/j.1476-5381.1972.tb07322.x.
A simple in vitro system was developed to study the effect of gamma-hydroxybutyrate on nerve cell depolarization-induced release of labelled DA and 5-hydroxytryptamine. The release of (3)H-dopamine formed in rat striatal slices incubated with (3)3H-tyrosine was followed. A three minute exposure to K+ (53.0 mM) caused a thirty-fold increase in the release of newly synthesized (3)H-dopamine. This K + -induced release was antagonized when gamma-hydroxybutyrate (1 mM) was present in the medium. Potassium (53.0 mM) increased (eighteen-fold) the release of (3)H-dopamine from striatal slices initially loaded by preincubation with (3)H-dopamine. The K + -induced release of this pool of DA was, however, not antagonized by gamma-hydroxybutyrate.Potassium (53.0 mM) also increased the release from striatal slices of (3)H-5-hydroxytryptamine (5-HT) newly synthesized from (3)H-tryptophan. This K + -induced release of 5-HT was also not inhibited by gamma-hydroxybutyrate. The ability of gamma-hydroxybutyrate to antagonize only the K + -induced release of newly formed DA may explain why this agent causes a rapid and selective increase in brain dopamine.
开发了一种简单的体外系统，以研究γ-羟基丁酸对神经细胞去极化诱导的标记多巴胺（DA）和5-羟色胺释放的影响。追踪了与（3）H-酪氨酸孵育的大鼠纹状体切片中形成的（3）H-多巴胺的释放。暴露于K+（53.0 mM）三分钟导致新合成的（3）H-多巴胺释放增加了30倍。当培养基中存在γ-羟基丁酸（1 mM）时，这种K +诱导的释放受到拮抗。钾（53.0 mM）使预先用（3）H-多巴胺预孵育而最初加载的纹状体切片中（3）H-多巴胺的释放增加（18倍）。然而，γ-羟基丁酸并未拮抗K +诱导的这部分DA的释放。钾（53.0 mM）还增加了由（3）H-色氨酸新合成的（3）H-5-羟色胺（5-HT）从纹状体切片中的释放。γ-羟基丁酸仅拮抗K +诱导的新形成的DA释放的能力，可能解释了为什么这种药物会导致脑多巴胺迅速且选择性地增加。
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