Saeki K, Yokoyama J, Wake K
J Pharmacol Exp Ther. 1975 Jun;193(3):910-7.
Granulomas were induced in rats by subcutaneous implantation of formalin-soaked filter-paper disks. Daily subcutaneous injection of histamine at doses of two times 0.05 mg/kg and above inhibited the growth of granulation tissue as measured by a marked decrease in the dry-defatted granuloma weight and of the hydroxyproline and hexosamine content. Histological observations of granulation tissue indicated that histamine inhibited the proliferation of fibroblasts and the formation of capillaries. Inhibitory effects were also observed with the histamine releaser, sinomenine, and the histaminase inhibitor, aminoguanidine. These histamine effects seem not to be mediated by glucocorticoid release, since an effective dose level of histamine produced no change in growth or thymus weight. Prednisolone was less potent than histamine in inhibiting Prednisolone was ineffective at the dose tested. Subcutaneous injection of the H2-receptor antagonist, burimamide, blocked these histamine effects and also of sinomeinine and aminoguanidine. The H1-receptor antagonist, mepyramine, did not block these histamine effects. Burimamide alone enhanced the growth of granuloma. These results indicate that granulation-tissue growth in inflammation is affected by the inhibitory effect of endogenous histamine acting through H2-receptors.
通过皮下植入福尔马林浸泡过的滤纸圆盘在大鼠体内诱导形成肉芽肿。每日皮下注射剂量为0.05mg/kg及以上的组胺两次，可抑制肉芽组织生长，表现为脱脂干肉芽肿重量、羟脯氨酸和己糖胺含量显著降低。肉芽组织的组织学观察表明，组胺抑制成纤维细胞增殖和毛细血管形成。组胺释放剂青藤碱和组胺酶抑制剂氨基胍也观察到了抑制作用。这些组胺作用似乎不是由糖皮质激素释放介导的，因为有效剂量的组胺对生长或胸腺重量没有影响。泼尼松龙在抑制方面的效力低于组胺，在所测试的剂量下无效。皮下注射H2受体拮抗剂布立马胺可阻断这些组胺作用，也可阻断青藤碱和氨基胍的作用。H1受体拮抗剂美吡拉敏不能阻断这些组胺作用。单独使用布立马胺可促进肉芽肿生长。这些结果表明，炎症中肉芽组织的生长受内源性组胺通过H2受体发挥的抑制作用影响。
