吖啶染料与噬菌体紧密堆积的DNA之间的相互作用。
The interaction of acridine dyes with the densely packed DNA of bacteriophage.
作者信息
Dusenbery D B, Uretz R B
出版信息
Biophys J. 1972 Aug;12(8):1056-65. doi: 10.1016/S0006-3495(72)86143-5.
Abstract
The interactions of acridine dyes with intact phage DNA differ from those with extracted DNA in the following respects. Strong binding (intercalation) is greatly reduced in intact phage but probably not eliminated. The cooperative, weak binding is stronger and the stacking tendency is increased. In gels of DNA the stacking tendency is seen to increase with decreasing hydration. These influences of the dense packing of DNA must be taken into account when using basic dyes to study chromosome structure.
摘要
吖啶染料与完整噬菌体DNA的相互作用在以下方面与它们和提取的DNA的相互作用有所不同。在完整噬菌体中，强结合（嵌入）作用大大降低，但可能并未消除。协同性的弱结合作用更强，且堆积倾向增加。在DNA凝胶中，堆积倾向会随着水合作用的降低而增加。在使用碱性染料研究染色体结构时，必须考虑到DNA紧密堆积的这些影响。
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