利用偏振荧光显微镜技术对几种直翅目昆虫成熟精子中的DNA组织进行研究。
DNA organization in the mature sperm of several orthoptera by the method of polarized fluorescence microscopy.
作者信息
Macinnes J W, Uretz R B
出版信息
J Cell Biol. 1968 Aug;38(2):426-36. doi: 10.1083/jcb.38.2.426.
Abstract
Mature sperm of Acheta domesticus, Acheta assimilis, Nemobius fasciatus, Nemobius confusus, Melanoplus femur-rubrum, Romalea microptera, Scudderia curvicauda, and Ceuthophilus nigricans were examined for DNA configuration by means of polarized fluorescence microscopy. In all cases, the results suggest that the DNA lies in unsupercoiled array, predominantly parallel to the elongate sperm head axes. Detailed calculations of the factors influencing the degree of polarization of the fluorescent emission from supercoiled DNA are contained in an appendix.
摘要
通过偏振荧光显微镜检查了家蟋蟀、拟家蟋蟀、条纹暗蝗、混淆暗蝗、红腿蝗、美洲蜚蠊、弯尾草螽和黑隐螽的成熟精子的DNA构型。在所有情况下，结果表明DNA呈非超螺旋排列，主要平行于细长的精子头部轴线。附录中包含了对影响超螺旋DNA荧光发射偏振程度的因素的详细计算。
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DNA organization in the mature sperm of several orthoptera by the method of polarized fluorescence microscopy.利用偏振荧光显微镜技术对几种直翅目昆虫成熟精子中的DNA组织进行研究。
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Birefringence of spermatozoa. I. Birefringence melting of squid, bull, and human sperm nucleoprotein.精子的双折射。I. 鱿鱼、公牛和人类精子核蛋白的双折射熔解
J Cell Biol. 1972 Nov;55(2):489-500. doi: 10.1083/jcb.55.2.489.
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The interaction of acridine dyes with the densely packed DNA of bacteriophage.吖啶染料与噬菌体紧密堆积的DNA之间的相互作用。
Biophys J. 1972 Aug;12(8):1056-65. doi: 10.1016/S0006-3495(72)86143-5.
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The orientation of DNA within 80-Angstrom chromatin fibers.80埃染色质纤维内DNA的取向。
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本文引用的文献
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Intrinsic Birefringence of Multiple-Coiled DNA, Theory and Applications.多螺旋DNA的固有双折射：理论与应用
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THE ARRANGEMENT OF CHROMOSOMES IN THE MATURE SPERM OF THE GRASSHOPPER.蝗虫成熟精子中染色体的排列
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Spectral analysis of the binding of acridine orange to polytene chromosomes.吖啶橙与多线染色体结合的光谱分析。
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Changes in deoxyribonucleoprotein during spermiogenesis in the bull.公牛精子发生过程中脱氧核糖核蛋白的变化
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