Croker K P, Robinson T J, Shelton J N
J Reprod Fertil. 1975 Jul;44(1):11-23. doi: 10.1530/jrf.0.0440011.
Two split-plot factorial experiments are described, the first with 72 entire cyclic ewes and the second with 80. The pattern of transport of spermatozoa through the reproductive tract was studied, following treatments with progestagen and oestrogen or with oestrogen alone during 2 weeks preceding insemination. A daily dose of 25 mug oestradiol-17 beta administered to ewes for 14 days preceeding oestrus had a deleterious effect on the passage of spermatozoa through the cervix into the uterus within the first 2 hr after insemination. The numbers of spermatozoa recoverable from the cranial region of the cervix 2 hr after insemination appeared to be related to the numbers in the oviducts at 24 hr. These numbers were related to fertility data from an earlier experiment using similar treatments. The data for log numbers of spermatozoa recoverable from the cervix formed a near-normal distribution and so were suitable for formal statistical analysis. There was an interaction between progestagen and oestrogen influence before mating on the pattern of sperm transport through the cervix.
本文描述了两项裂区析因试验，第一项试验有72只完整的周期性母羊，第二项试验有80只。研究了在人工授精前2周用孕激素和雌激素或仅用雌激素处理后，精子在生殖道中的运输模式。在发情前14天给母羊每日注射25微克雌二醇-17β，对人工授精后最初2小时内精子通过子宫颈进入子宫的过程有有害影响。人工授精后2小时从子宫颈头部区域可回收的精子数量似乎与24小时时输卵管中的精子数量有关。这些数量与早期使用类似处理的试验中的生育力数据有关。从子宫颈可回收的精子对数数据呈近似正态分布，因此适合进行正式的统计分析。交配前孕激素和雌激素对精子通过子宫颈的运输模式的影响存在交互作用。
