Parker K R, Mant M J
Thromb Haemost. 1979 Aug 31;42(2):743-51.
The saliva of the tsetse, Glossina morsitans morsitans Westwood, has antithrombin anticoagulant activity and inhibits thrombin's esterolytic activity. It has no other detectable anticoagulant properties. The anticoagulant elutes in a single peak on Sephadex fraction, is immediately acting, heat and storage stable, and has a molecular weight of 11-13,000. Unlike heparin it is not neutralized by protamine sulphate or toluidine blue and does not require the co-factor, antithrombin III, for optimal anticoagulant activity. It has similar properties to hirudin, but does not elute with a protein peak upon Sephadex fractionation and has a slightly different molecular weight. Salivary gland homogenates contained neither a plasminogen activator nor fibrinolytic activity. The sera of rabbits used to maintain tsetses, which contained precipitating antibodies against saliva, did not neutralize the salivary anticoagulant in vitro. The properties of this anticoagulant suggest that it might be a potentially useful antithrombotic agent in man.
采采蝇（Glossina morsitans morsitans Westwood）的唾液具有抗凝血酶抗凝活性，并能抑制凝血酶的酯解活性。它没有其他可检测到的抗凝特性。该抗凝剂在葡聚糖凝胶柱上以单一峰洗脱，作用迅速，对热和储存稳定，分子量为11000 - 13000。与肝素不同，它不会被硫酸鱼精蛋白或甲苯胺蓝中和，且最佳抗凝活性不需要辅助因子抗凝血酶III。它与水蛭素具有相似的特性，但在葡聚糖凝胶分级分离时不会与蛋白质峰一起洗脱，分子量也略有不同。唾液腺匀浆既不含纤溶酶原激活剂，也没有纤溶活性。用于饲养采采蝇的兔血清中含有针对唾液的沉淀抗体，但在体外不能中和唾液抗凝剂。这种抗凝剂的特性表明它可能是一种对人类潜在有用的抗血栓形成药物。
