Arens M, Schaeg W, Krauss H
Zentralbl Bakteriol Orig A. 1979 Jul;244(2-3):309-15.
By use of 6-molar guanidinium chloride a potent Coxiella burnetii antigen could be produced for diagnostic purposes from infected yolk sacs, with little technical expense. This treatment did not only a cause remarkably purifying effect (Tab. 1) but also the extraction of soluble cytoplasmic substance. From guanidinium chloride-treated suspensions a highly purified and uniform suspension of cell walls could be separated by Saccharose Density Gradient centrifugation (Fig. 2). Guanidine extracted organisms retained their full antigenic potential with respect to Phase I and Phase II and lacked anticomplementary activity. Such preparations can be used for serological tests like complement fixation reaction or Enzyme Linked Immunosorbent Assay and are particularly suitable for biochemical studies of Phase antigens of Coxiella burnetii.
通过使用6摩尔的氯化胍，可从感染的卵黄囊中制备出用于诊断目的的强效伯氏考克斯氏体抗原，且技术成本很低。这种处理不仅产生了显著的纯化效果（表1），还提取了可溶性细胞质物质。通过蔗糖密度梯度离心法（图2），可以从经氯化胍处理的悬浮液中分离出高度纯化且均匀的细胞壁悬浮液。胍提取的生物体在I相和II相方面保留了其全部抗原潜力，并且缺乏抗补体活性。此类制剂可用于补体结合反应或酶联免疫吸附测定等血清学检测，特别适用于伯氏考克斯氏体相抗原的生化研究。
