Quevedo Diaz M A, Lukácová M
Institute of Virology, Bratislava, Slovak Republic.
Acta Virol. 1998 Jun;42(3):181-5.
The influence of the number of passages in chick embryo yolk sac (EPs) on the properties of the lipopolysaccharide (LPS) and other antigens of Coxiella burnetii Priscilla strain in polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), immunoblot analysis, enzyme-linked immunosorbent assay (ELISA) and complement-fixation reaction (CFR) test has been studied. Three phases in the phase variation of Coxiella burnetii could be distinguished by these methods: phase I lasting up to the 20th passage (EP 20), intermediate phase corresponding to EP 20-EP 70, and phase II beginning at EP 80. The changes in LPS were more marked than those in proteins which conserved their immunoblot profile up to EP 80. The phase II was clearly demonstrated by all the methods used.
研究了鸡胚卵黄囊传代次数（EPs）对贝氏柯克斯体普里西拉菌株脂多糖（LPS）及其他抗原在十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS - PAGE）、免疫印迹分析、酶联免疫吸附测定（ELISA）和补体结合反应（CFR）试验中特性的影响。通过这些方法可区分贝氏柯克斯体相变的三个阶段：I期持续到第20代传代（EP 20），中间期对应EP 20 - EP 70，II期从EP 80开始。LPS的变化比蛋白质更明显，蛋白质在传代至EP 80时仍保持其免疫印迹图谱。所用的所有方法都清楚地证明了II期。
