Pfaller K, Schuler G, Schmidt W, Dworschak K
Anat Anz. 1979;145(4):404-12.
Investigation of in situ fixed skin clearly showed distinct arrangement and orientation of collagenous fibre bundles clearly visible only after in situ fixation of whole bodies. Light microscopy seems especially useful to study gross fibre and fibre bundle arrangement, whereas scanning electron microscopy is better suited to study the finer details of fibres and fibre bundles and their arrangement. 2 main directions of fibre bundles were found. The orientation was corresponding to the main mechanical stresses exerted to the skin. No clear start and ending of the fibre bundles could be distinguished. They seem to be arranged in a 3 dimensional texture. Fibres are branching off from one bundle and merge again either with a branch of the same bundle or with a branch of another bundle, in a higher or a lower layer of this 3 dimensional texture. In addition to the 2 main directions of the bundle orientation thin fibres are penetrating the whole texture in various directions. This is especially prominent in regions where vessels, hairbulbs or glands are protruding into the stratum reticulare. A quantitative study of fibre and fibre bundle orientation using semithin sections is under current investigation applying an automatic image analysis system.
对原位固定皮肤的研究清楚地显示，胶原纤维束有明显的排列和方向，只有在对整个身体进行原位固定后才能清晰可见。光学显微镜似乎特别有助于研究粗大纤维和纤维束的排列，而扫描电子显微镜更适合研究纤维和纤维束及其排列的更细微细节。发现了纤维束的两个主要方向。其方向与施加于皮肤的主要机械应力相对应。无法区分纤维束的明确起点和终点。它们似乎以三维结构排列。纤维从一个纤维束分支出来，然后在这个三维结构的较高或较低层中，再次与同一纤维束的一个分支或另一个纤维束的一个分支合并。除了纤维束方向的两个主要方向外，细纤维还在各个方向穿透整个结构。这在血管、毛囊或腺体突入网状层的区域尤为突出。目前正在使用自动图像分析系统，对使用半薄切片的纤维和纤维束方向进行定量研究。
