大鼠肺脏体外脂质代谢。正常及饥饿大鼠对柠檬酸的利用。
Lipid metabolism by rat lung in vitro. Utilization of citrate by normal and starved rats.
作者信息
Scholz R W
出版信息
Biochem J. 1972 Mar;126(5):1219-24. doi: 10.1042/bj1261219.
Abstract
- The utilization of [1,5-(14)C(2)]citrate by lung slices and cell cytosol preparations, and the activities of liver and lung cytosol citrate-cleavage enzyme (EC 4.1.3.8), l-malate-NAD oxidoreductase (malate dehydrogenase, EC 1.1.1.37) and phosphoenolpyruvate carboxylase (EC 4.1.1.32) were examined in normal and starved rats. 2. Lipogenesis from citrate was decreased by approx. 70% in both the phospholipid and neutral lipid fractions of lung slices from starved rats as compared with fed controls. 3. Incorporation of citrate by lung cytosol preparations into fatty acids was decreased by approx. 35% in the starved rats. The apparent inhibition by avidin of fatty acid synthesis was overcome partially by preincubation of lung cytosol preparations with biotin. These results are consistent with the presence in lung tissue of the malonyl-CoA pathway for fatty acid synthesis. 4. Lung citrate-cleavage enzyme activity decreased in rats that had been starved for 72h whereas malate dehydrogenase and phosphoenolpyruvate carboxylase activities remained unchanged. The results suggest that the pattern of utilization of lipid precursors by rat lung may be altered during various nutritional states.
摘要
- 研究了正常和饥饿大鼠肺切片及细胞胞质溶胶制剂对[1,5-(14)C₂]柠檬酸的利用情况，以及肝和肺胞质溶胶柠檬酸裂解酶（EC 4.1.3.8）、L-苹果酸-NAD氧化还原酶（苹果酸脱氢酶，EC 1.1.1.37）和磷酸烯醇丙酮酸羧化酶（EC 4.1.1.32）的活性。2. 与喂食对照相比，饥饿大鼠肺切片的磷脂和中性脂质部分中，由柠檬酸生成脂肪的过程减少了约70%。3. 饥饿大鼠肺胞质溶胶制剂将柠檬酸掺入脂肪酸的量减少了约35%。用生物素预孵育肺胞质溶胶制剂可部分克服抗生物素蛋白对脂肪酸合成的明显抑制作用。这些结果与肺组织中存在脂肪酸合成的丙二酰辅酶A途径一致。4. 饥饿72小时的大鼠肺柠檬酸裂解酶活性降低，而苹果酸脱氢酶和磷酸烯醇丙酮酸羧化酶活性保持不变。结果表明，在不同营养状态下，大鼠肺对脂质前体的利用模式可能会发生改变。
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