通过亲和色谱法分离茎菠萝蛋白酶及其通过凝胶电泳进行的部分表征。 - Suppr | 超能文献

Isolation of stem bromelain by affinity chromatography and its partial characterization by gel electrophoresis.

作者信息

Bobb D

出版信息

Prep Biochem. 1972;2(4):347-54. doi: 10.1080/00327487208065671.

DOI:10.1080/00327487208065671

Abstract

摘要

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Isolation of stem bromelain by affinity chromatography and its partial characterization by gel electrophoresis.通过亲和色谱法分离茎菠萝蛋白酶及其通过凝胶电泳进行的部分表征。

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The structure and mechanism of stem bromelain. Evaluation of the homogeneity of purified stem bromelain, determination of the molecular weight and kinetic analysis of the bromelain-catalysed hydrolysis of N-benzyloxycarbonyl-L-phenylalanyl-L-serine methyl ester.茎菠萝蛋白酶的结构与机制。纯化茎菠萝蛋白酶的均一性评估、分子量测定以及菠萝蛋白酶催化N-苄氧羰基-L-苯丙氨酰-L-丝氨酸甲酯水解的动力学分析。

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