Davalli P, Ligabue A, Guarnieri C
Boll Soc Ital Biol Sper. 1979 May 30;55(10):925-31.
The incorporation of 3H-tyrosine into norepinephrine of rabbit hearts perfused with Krebs-Henseleit solution containing 11 mM glucose and gassed with 95% O2 - 5% CO2 (control) or 80% O2 - 5% CO2 15% N2 (hypoxic) or 95% N2 - 5% CO2 (anoxic), was studied. In the control hearts a constant specific activity of norepinephrine without any release of catecholamine into coronary effluent was measured after 5, 15, 30, 60 minutes. The hypoxic perfusion while not causing any release of norepinephrine, produced a significant increase in tyrosine incorporation into norepinephrine. The specific activity of norepinephrine and its release into coronary effluent were increased by anoxic perfusion; this effect was most evident when the glucose was replaced with a solution containing 11 mM mannitol. These results suggest that the synthesis of norepinephrine in isolated hearts, lacking of sympathetic innervation, may be affected by different condition of oxygen supply.
研究了在含11 mM葡萄糖的Krebs - Henseleit溶液中灌注，并分别用95% O₂ - 5% CO₂（对照）、80% O₂ - 5% CO₂ - 15% N₂（低氧）或95% N₂ - 5% CO₂（缺氧）气体饱和的情况下，3H - 酪氨酸掺入兔心脏去甲肾上腺素的情况。在对照心脏中，5、15、30、60分钟后测量到去甲肾上腺素的比活性恒定，且没有儿茶酚胺释放到冠状动脉流出液中。低氧灌注虽然不会导致去甲肾上腺素的任何释放，但会使酪氨酸掺入去甲肾上腺素的量显著增加。缺氧灌注会增加去甲肾上腺素的比活性及其释放到冠状动脉流出液中的量；当用含11 mM甘露醇的溶液代替葡萄糖时，这种效应最为明显。这些结果表明，在缺乏交感神经支配的离体心脏中，去甲肾上腺素的合成可能会受到不同供氧条件的影响。
