通过二磷酸腺苷的硫代磷酸化酶促制备腺苷5'-O-(3-[35S]硫代三磷酸)
Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate.
作者信息
Cassidy P S, Kerrick W G
出版信息
Biochim Biophys Acta. 1979 Nov 22;565(1):209-13. doi: 10.1016/0005-2787(79)90097-2.
PMID:508762
Abstract
The very useful radiolabeled ATP analog, adenosine 5-O-(3-[35S] thiotriphosphate) or [35S] ATPgammaS, has been prepared by a technique based on the thiophosphorylation of ADP that results in much higher yields of [35S] ATPgammaS than does a thiophosphate exchange method [1].
摘要
非常有用的放射性标记的ATP类似物，即腺苷5-O-(3-[35S]硫代三磷酸)或[35S]ATPγS，已通过一种基于ADP硫代磷酸化的技术制备而成，该技术所产生的[35S]ATPγS产量比硫代磷酸交换法高得多[1]。
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