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通过二磷酸腺苷的硫代磷酸化酶促制备腺苷5'-O-(3-[35S]硫代三磷酸)

Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate.

作者信息

Cassidy P S, Kerrick W G

出版信息

Biochim Biophys Acta. 1979 Nov 22;565(1):209-13. doi: 10.1016/0005-2787(79)90097-2.

DOI:10.1016/0005-2787(79)90097-2
PMID:508762
Abstract

The very useful radiolabeled ATP analog, adenosine 5-O-(3-[35S] thiotriphosphate) or [35S] ATPgammaS, has been prepared by a technique based on the thiophosphorylation of ADP that results in much higher yields of [35S] ATPgammaS than does a thiophosphate exchange method [1].

摘要

非常有用的放射性标记的ATP类似物，即腺苷5-O-(3-[35S]硫代三磷酸)或[35S]ATPγS，已通过一种基于ADP硫代磷酸化的技术制备而成，该技术所产生的[35S]ATPγS产量比硫代磷酸交换法高得多[1]。

相似文献

1
Enzymatic preparation of adenosine 5'-O-(3-[35S]thiotriphosphate) by thiophosphorylation of adenosine diphosphate.通过二磷酸腺苷的硫代磷酸化酶促制备腺苷5'-O-(3-[35S]硫代三磷酸)
Biochim Biophys Acta. 1979 Nov 22;565(1):209-13. doi: 10.1016/0005-2787(79)90097-2.
2
Enzymatic synthesis of adenosine 5'-O-(3-[35S]-thiotriphosphate).
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Thiophosphorylation of (Na + K+)-ATPase yields an ADP-sensitive phosphointermediate.
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Synthesis and properties of diastereoisomers of adenosine 5'-(O-1-thiotriphosphate) and adenosine 5'-(O-2-thiotriphosphate).腺苷5'-(O-1-硫代三磷酸酯)和腺苷5'-(O-2-硫代三磷酸酯)非对映异构体的合成与性质
Biochemistry. 1976 Apr 20;15(8):1685-91. doi: 10.1021/bi00653a015.

引用本文的文献

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Pflugers Arch. 1981 Dec;392(2):121-4. doi: 10.1007/BF00581259.
2
Inhibition of Ca2+-activated tension and myosin light chain phosphorylation in skinned smooth muscle strips by the phenothiazines.吩噻嗪类药物对去表皮平滑肌条中钙激活张力和肌球蛋白轻链磷酸化的抑制作用。
Pflugers Arch. 1980 Sep;387(2):115-20. doi: 10.1007/BF00584261.
3
The accuracy of protein biosynthesis is limited by its speed: high fidelity selection by ribosomes of aminoacyl-tRNA ternary complexes containing GTP[gamma S].
蛋白质生物合成的准确性受其速度限制：核糖体对含有鸟苷三磷酸γ-硫代物（GTP[γS]）的氨酰-tRNA三元复合物进行高保真选择。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4922-6. doi: 10.1073/pnas.79.16.4922.
4
Calcium-regulatory mechanisms. Functional classification using skinned fibers.钙调节机制。使用剥除肌膜纤维的功能分类。
J Gen Physiol. 1981 Feb;77(2):177-90. doi: 10.1085/jgp.77.2.177.
5
MgADP- increases maximum tension and Ca2+ sensitivity in skinned rabbit soleus fibers.MgADP-可增加去表皮兔比目鱼肌纤维的最大张力和Ca2+敏感性。
Pflugers Arch. 1987 Sep;410(1-2):30-6. doi: 10.1007/BF00581892.