姐妹染色单体差异染色与吉姆萨显带相结合。
A combination of sister chromatid differential staining and giemsa banding.
作者信息
Pathak S, Stock A D, Lusby A
出版信息
Experientia. 1975 Aug 15;31(8):916-8. doi: 10.1007/BF02358850.
PMID:50952
Abstract
We report a procedure for combining sister chromatid differential staining and G banding in the same metaphase plate. Mammalian cells in culture are grown in medium containing 5-bromodeoxyuridine for two cell cycles, and conventional air-dried preparations are made. The slides are treated with a trypsin or a urea solution the same way as for regular G banding. This method is simple and fast and provides additional information for cytogeneticists.
摘要
我们报告了一种在同一中期板上结合姐妹染色单体差异染色和G带染色的方法。培养中的哺乳动物细胞在含有5-溴脱氧尿苷的培养基中生长两个细胞周期，然后制作常规的空气干燥标本。玻片用胰蛋白酶或尿素溶液处理，方法与常规G带染色相同。该方法简单快速，为细胞遗传学家提供了额外的信息。
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