Shimizu T, Nitta M, Mifuchi I
Gan. 1979 Aug;70(4):429-33.
Antitumor activity of marine bacteria, Vibrio anguillarum P-B-1, against Ehrlich carcinoma cells in ddY mice was investigated. Ehrlich carcinoma cells were inoculated intraperitoneally (ip) into mice and V. anguillarum (1.0 mg/mouse) was administered ip twice before and 4 times after the tumor inoculation. Groups of mice administered V. anguillarum survived 80 approximately 90% and their mean survival was 54.0 approximately 56.0 days (range, 24.0 approximately 60 days) at day 60 after inoculation of 10(4) to 10(6) tumor cells against the mean survival of 16.4 approximately 22.3 days (range, 7 approximately 26) in the control group. When 2 x 10(6) tumor cells were inoculated subcutaneously (sc) mixed with 1.0 mg of V. anguillarum, the bacterial cells markedly suppressed the growth of tumor at the injection site; tumor did not grow in 30% of recipient mice and the inhibition rate of grown tumor in the rest of recipients was 69%. The consistently demonstrable antitumor activity of V. anguillarum was reduced by pretreatment of mice with immunosuppressants such as anti-thymocyte serum, hydrocortisone, or irradiation of X-ray. This fact indicates that the antitumor activity of V. anguillarum is mediated by immune response.
研究了海洋细菌鳗弧菌P - B - 1对ddY小鼠艾氏癌细胞的抗肿瘤活性。将艾氏癌细胞腹腔注射接种到小鼠体内，在肿瘤接种前腹腔注射鳗弧菌（1.0mg/小鼠）2次，接种后腹腔注射4次。接种10⁴至10⁶个肿瘤细胞后60天，给予鳗弧菌的小鼠组约80%至90%存活，其平均存活时间为54.0至56.0天（范围为24.0至60天），而对照组的平均存活时间为16.4至22.3天（范围为7至26天）。当皮下注射2×10⁶个肿瘤细胞并与1.0mg鳗弧菌混合时，细菌细胞显著抑制了注射部位肿瘤的生长；30%的受体小鼠肿瘤未生长，其余受体小鼠中已生长肿瘤的抑制率为69%。用抗胸腺细胞血清、氢化可的松等免疫抑制剂预处理小鼠或进行X射线照射后，鳗弧菌持续可证明的抗肿瘤活性降低。这一事实表明，鳗弧菌的抗肿瘤活性是由免疫反应介导的。
