Shimizu T, Itoh Y, Mifuchi I, Iguchi T, Kondo S, Hisatsune K
Gan. 1983 Apr;74(2):279-84.
The antitumor activity of a marine bacterium, Vibrio anguillarum, against Ehrlich carcinoma cells in ddY mice was investigated. The aqueous layer obtained by the hot phenol-water procedure exhibited more antitumor activity than did the middle layer or the phenol layer. This finding indicates that lipopolysaccharide (LPS) derived from V. anguillarum exhibits significant antitumor activity. In fact, mice injected with LPS obtained by ultracentrifugation and treatment with RNase had a longer mean survival period than the control mice. V. anguillarum LPS also inhibited the growth of syngeneic fibrosarcoma induced by 3-methylcholanthrene in C57BL/6 mice. V. anguillarum LPS possesses no 2-keto-3-deoxyoctonate, a regular sugar component of the core region of most gram-negative bacterial LPS, suggesting that 2-keto-3-deoxyoctonate is unnecessary for the antitumor activity of LPS.
研究了海洋细菌鳗弧菌对ddY小鼠艾氏癌细胞的抗肿瘤活性。通过热酚 - 水法获得的水层比中层或酚层表现出更强的抗肿瘤活性。这一发现表明，源自鳗弧菌的脂多糖（LPS）具有显著的抗肿瘤活性。事实上，注射经超速离心和核糖核酸酶处理获得的LPS的小鼠比对照小鼠的平均存活期更长。鳗弧菌LPS还抑制了C57BL / 6小鼠中由3 - 甲基胆蒽诱导的同基因纤维肉瘤的生长。鳗弧菌LPS不含有2 - 酮 - 3 - 脱氧辛酸，而2 - 酮 - 3 - 脱氧辛酸是大多数革兰氏阴性细菌LPS核心区域的常规糖成分，这表明2 - 酮 - 3 - 脱氧辛酸对于LPS的抗肿瘤活性并非必需。
