Kataoka T, Tsukagoshi S, Sakurai Y, Okabe M
Gan. 1979 Aug;70(4):515-9.
Intraperitoneal inoculation of either levamisole or 2 x 10(6) cells of concanavalin-A (Con-A)-bound L1210 leukemia vaccine produced no cured mice after subsequent ip inoculation of 10(3) live L1210 cells. Combined inocluation of levamisole and Con-A-bound vaccine produced about 20% cure incidence but no prolongation of life span of tumor-bearing mice after inoculation of live L1210 cells. Combined inoculation of levamisole and Con A-free vaccine did not induce detectable immune resistance in mice. These results suggest that levamisole enhanced host response to cell-bound Con-A associated with immunogenic potency of the vaccine. Levamisole given intravenously and orally was as effective as that inoculated intraperitoneally in enhancing the induction of resistance in mice by Con-A-bound vaccine. Dose of levamisole was very critical for this enhancement, being effective at 0.38 mg/kg but not either at 0.75 or 0.19 mg/kg. Furthermore, levamisole restored immune resistance induced by a larger inoculum of Con-A-bound vaccine cells (10(7)) and impaired by cyclophosphamide.
腹腔内接种左旋咪唑或2×10⁶个伴刀豆球蛋白A（Con-A）结合的L1210白血病疫苗细胞后，随后腹腔内接种10³个活L1210细胞，未产生治愈的小鼠。左旋咪唑与Con-A结合疫苗联合接种产生了约20%的治愈率，但接种活L1210细胞后，荷瘤小鼠的寿命未延长。左旋咪唑与不含Con A的疫苗联合接种未在小鼠中诱导可检测到的免疫抗性。这些结果表明，左旋咪唑增强了宿主对与疫苗免疫原性相关的细胞结合Con-A的反应。静脉内和口服给予左旋咪唑在增强Con-A结合疫苗诱导小鼠抗性方面与腹腔内接种同样有效。左旋咪唑的剂量对这种增强作用非常关键，0.38mg/kg有效，而0.75mg/kg或0.19mg/kg均无效。此外，左旋咪唑恢复了由较大接种量（10⁷）的Con-A结合疫苗细胞诱导且被环磷酰胺损害的免疫抗性。
