Kataoka T, Oh-hashi F, Sakurai Y
Gan. 1979 Apr;70(2):155-64.
Glutaraldehyde-treated L1210 murine leukemic cells induced immune resistance in mice when concanavalin-A (Con-A) was bound to their surface. They were not immunogenic when Con-A was separately inoculated, indicating requirement of vaccine-bound Con-A for enhancing immunogenic potency of the vaccine. Based on the finding that vaccine-bound Con-A induced in vitro blastogenesis of spleen cells 10 times as efficiently as unbound Con-A did, the association of blastogenic potency of Con-A-bound vaccine with its immunogenic activity was examined. The possibility of their association was supported by the finding that induction of immune resistance by vaccines was reduced when their blastogenic activities had been reduced by treatment of the vaccine with anti-Con-A antisera or by substituting succinyl-Con-A or other lectins for Con-A. Moreover, the fact that combined inoculations of Con-A-bound vaccine, assumed to be blastogenic to T cells, with anti-thymocyte sera, but not with cyclophosphamide or carrageenan, resulted in reduced production of resistant animals was also consistent with, but not proving, this possibility. Feasibility of this possibility was further supported by the finding that Con-A-bound vaccine did not induce immune resistance in animals that had been abrogated in blastogenic activity of spleen cells by prior administration of Con-A.
戊二醛处理的L1210小鼠白血病细胞在其表面结合刀豆球蛋白A（Con-A）时可诱导小鼠产生免疫抗性。当单独接种Con-A时，它们没有免疫原性，这表明疫苗结合的Con-A是增强疫苗免疫原性效力所必需的。基于疫苗结合的Con-A体外诱导脾细胞增殖的效率是未结合的Con-A的10倍这一发现，研究了Con-A结合疫苗的增殖效力与其免疫活性之间的关联。当用抗Con-A抗血清处理疫苗或用琥珀酰-Con-A或其他凝集素替代Con-A以降低其增殖活性时，疫苗诱导的免疫抗性降低，这一发现支持了它们之间存在关联的可能性。此外，假定对T细胞具有增殖作用的Con-A结合疫苗与抗胸腺细胞血清联合接种，但不与环磷酰胺或角叉菜胶联合接种，导致产生抗性动物的数量减少，这一事实也与这种可能性相符，但并未证实。Con-A结合疫苗在预先给予Con-A使脾细胞增殖活性被消除的动物中不诱导免疫抗性，这一发现进一步支持了这种可能性的可行性。
