海胆沙钱胚胎发育过程中DNA的甲基化

Methylation of DNA in developing embryos of the sea urchin Psammechinus miliaris.

作者信息

Baur R, Wohlert H, Kröger H

出版信息

Hoppe Seylers Z Physiol Chem. 1979 Sep;360(9):1263-9. doi: 10.1515/bchm2.1979.360.2.1263.

DOI:10.1515/bchm2.1979.360.2.1263

Abstract

Embryos of the sea urchin Psammechinus miliaris have been labelled after fertilization with [6(-3)H]uridine and cultured in filtered sea water. 32-cell, blastula, gastrula and pluteus stages were harvested. The DNA from these embryos was purified and hydrolyzed and the nuclear bases were analyzed by means of high performance liquid chromatography. The ratios of 5-methylcytosine and cytosine demonstrate that the concentrations of 5-methylcytosine are essentially the same in the developmental stages examined (gamma = 95%), which contradicts the hypothesis that methylation of DNA plays a role in cell differentiation.

摘要

用[6(-3)H]尿苷在受精后对海胆粒刺海胆的胚胎进行标记，并在经滤过的海水中培养。收获了32细胞期、囊胚期、原肠胚期和羽腕幼虫期的胚胎。将这些胚胎的DNA进行纯化和水解，并通过高效液相色谱法分析核碱基。5-甲基胞嘧啶与胞嘧啶的比率表明，在所检测的发育阶段中5-甲基胞嘧啶的浓度基本相同（γ=95%），这与DNA甲基化在细胞分化中起作用的假说相矛盾。