Wagner H, Simon D, Werner E, Gelderblom H, Darai C, Flügel R M
J Virol. 1985 Mar;53(3):1005-7. doi: 10.1128/JVI.53.3.1005-1007.1985.
The content and distribution of 5-methylcytosine in DNA from fish lymphocystis disease virus was analyzed by high-pressure liquid chromatography, nearest-neighbor analysis, and with restriction endonucleases. We found that 22% of all C residues were methylated, including methylation of the following dinucleotide sequences: CpG to 75%, CpC to ca. 1%, and CpA to 2 to 5%. Comparison of relative digestion of viral DNA with MspI and HpaII indicated that CCGG sequences were almost completely methylated at the inner C. The degree of methylation of GCGC was much lower. The methylation pattern of fish lymphocystis disease virus DNA differed from that of the host cell DNA.
通过高压液相色谱法、邻位分析和限制性内切酶对鱼类淋巴囊肿病病毒DNA中5-甲基胞嘧啶的含量和分布进行了分析。我们发现，所有C残基中有22%被甲基化，包括以下二核苷酸序列的甲基化：CpG为75%，CpC约为1%，CpA为2%至5%。病毒DNA与MspI和HpaII相对消化率的比较表明，CCGG序列在内侧C处几乎完全甲基化。GCGC的甲基化程度要低得多。鱼类淋巴囊肿病病毒DNA的甲基化模式与宿主细胞DNA不同。
