Franklin C D, Craig G T, Smith C J
Histopathology. 1979 Nov;3(6):511-22. doi: 10.1111/j.1365-2559.1979.tb03032.x.
Stereological techniques have been used to compare histological parameters of the giant cell component in 10 giant cell tumours of long bones, 10 central giant cell granulomas of the jaws and 10 peripheral giant cell granulomas of the jaws. The giant cell parameters investigated were: mean profile diameter, mean diameter, mean profile axial ratio, volume-to-surface ratios, nuclear numerical density, profile area and cell volume. Serial sections were cut at 4 micron from routinely fixed, paraffin-embedded specimens of each case and two sections, separated by 100 micron, were stained with haematoxylin and eosin. Random fields were photographed until at least 25 giant cell profiles had been recorded from each of the two sections of each lesion. The processed film was projected for point and intersect counting, the entire procedure ensuring double-blind assessment of the cases under investigation. In general there was no significant difference for any of the parameters between the giant cells of central and peripheral jaw lesions. There was a significant difference, however, between the giant cells of central jaw lesions and long bone tumours in respect of both nuclear numerical density and mean absolute cell volume.
采用体视学技术比较了10例长骨巨细胞瘤、10例颌骨中央巨细胞肉芽肿和10例颌骨外周巨细胞肉芽肿中巨细胞成分的组织学参数。所研究的巨细胞参数包括：平均轮廓直径、平均直径、平均轮廓轴比、体积与表面积比、核数值密度、轮廓面积和细胞体积。从每个病例常规固定、石蜡包埋的标本上切取4微米厚的连续切片，每隔100微米取两片，用苏木精和伊红染色。拍摄随机视野，直到从每个病变的两片切片中至少记录到25个巨细胞轮廓。将处理后的胶片投影进行点计数和交叉计数，整个过程确保对所研究的病例进行双盲评估。一般来说，颌骨中央和外周病变的巨细胞在任何参数上均无显著差异。然而，颌骨中央病变的巨细胞与长骨肿瘤的巨细胞在核数值密度和平均绝对细胞体积方面存在显著差异。
