含二氧化硫发酵液中硫化氢的测定
Determination of hydrogen sulfide in fermentation broths containing SO 2 .
作者信息
Acree T E, Sonoff E P, Splittstoesser D F
出版信息
Appl Microbiol. 1971 Jul;22(1):110-2. doi: 10.1128/am.22.1.110-112.1971.
Abstract
A procedure for the determination of hydrogen sulfide in fermentation broths containing up to 100 mug of SO(2) per ml is described. The method involves the sparging of H(2)S from the broth into a cadmium hydroxide absorption solution, the formation of methylene blue from the absorbed sulfide, and the measuring of this color spectrophotometrically. The use of cadmium hydroxide instead of zinc acetate, the common absorbent, substantially reduced the interference of SO(2) with the analysis.
摘要
本文描述了一种用于测定每毫升含高达100微克SO₂的发酵液中硫化氢的方法。该方法包括将发酵液中的H₂S鼓泡到氢氧化镉吸收溶液中，使吸收的硫化物形成亚甲基蓝，并通过分光光度法测量该颜色。使用氢氧化镉代替常用的吸收剂醋酸锌，大大减少了SO₂对分析的干扰。
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