von Metz E, Eisler D M, Hottle G A
Appl Microbiol. 1971 Jul;22(1):84-8. doi: 10.1128/am.22.1.84-88.1971.
The median effective doses (ED(50)) of 28 lots of killed Pasteurella pestis strain 195/P vaccine were determined in mice and guinea pigs. Mice were injected with vaccine alone, whereas guinea pigs received vaccine suspended in incomplete Freund's adjuvant. Potency ratios of vaccines were obtained by comparing the ED(50) of the test with that of a reference vaccine. Mean potency ratios of 1.82 +/- 0.50 in mice and 3.22 +/- 0.56 in guinea pigs were obtained, and the difference between these means was significant, P = <0.01. The number of organisms in the challenge dose did not significantly affect the ED(50) of a vaccine in guinea pigs. However, irrespective of vaccinating route, nearly 1,000 times as much vaccine was required in the absence of adjuvant as in its presence to produce comparable protective indexes in the guinea pig. The response of guinea pigs did not offer any improvement over mice in evaluating the efficacy of plague vaccines.
测定了28批鼠疫杆菌195/P株灭活疫苗在小鼠和豚鼠体内的半数有效剂量（ED50）。小鼠单独注射疫苗，而豚鼠接种用不完全弗氏佐剂悬浮的疫苗。通过比较试验疫苗与参比疫苗的ED50获得疫苗效价比。在小鼠中获得的平均效价比为1.82±0.50，在豚鼠中为3.22±0.56，这些平均值之间的差异具有显著性，P＜0.01。攻击剂量中的菌数对豚鼠体内疫苗的ED50没有显著影响。然而，无论接种途径如何，在没有佐剂的情况下，要在豚鼠中产生可比的保护指数，所需疫苗量几乎是有佐剂时的1000倍。在评估鼠疫疫苗的效力方面，豚鼠的反应并不比小鼠有任何优势。
