溴化乙锭对HeLa细胞线粒体DNA合成及线粒体DNA结构的影响。
The effect of ethidium bromide on mitochondrial DNA synthesis and mitochondrial DNA structure in HeLa cells.
作者信息
Leibowitz R D
出版信息
J Cell Biol. 1971 Oct;51(1):116-22. doi: 10.1083/jcb.51.1.116.
Abstract
The synthesis of mitochondrial DNA (mDNA) in HeLa cells is selectively inhibited by relatively low concentrations of ethidium bromide. After exposure of cells to strongly inhibitory concentrations of the drug, the apparent superhelix density of mDNA is rapidly increased, as judged by its buoyant density in CsCl in the presence of ethidium bromide. Mitochondrial DNA synthesized in the presence of partially inhibitory concentrations of ethidium bromide is also altered in its buoyant density in the presence of the dye, but is more heterogeneous in this respect. However, the change in buoyant density of newly synthesized mDNA may be explained by changes in structure other than a change in superhelix density, as indicated by its increased resistance to digestion by pancreatic DNase.
摘要
相对低浓度的溴化乙锭可选择性抑制海拉细胞中线粒体DNA（mDNA）的合成。在细胞暴露于该药物的强抑制浓度后，根据其在溴化乙锭存在下于CsCl中的浮力密度判断，mDNA的表观超螺旋密度迅速增加。在部分抑制浓度的溴化乙锭存在下合成的线粒体DNA，在该染料存在时其浮力密度也会改变，但在这方面更具异质性。然而，新合成的mDNA浮力密度的变化可能是由超螺旋密度变化以外的结构变化所解释的，这一点可由其对胰DNA酶消化的抗性增加表明。
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