伴刀豆球蛋白A与人分泌物中血型物质糖蛋白的相互作用。
The interaction of concanavalin A with blood-group-substance glycoproteins from human secretions.
作者信息
Clarke A E, Denborough M A
出版信息
Biochem J. 1971 Mar;121(5):811-6. doi: 10.1042/bj1210811.
Abstract
- Gastric juice, saliva and ovarian-cyst fluid were fractionated into glycoprotein components by centrifuging to equilibrium in a caesium chloride density gradient. 2. The glycoprotein fractions from the gastric juice of two group O non-secretors, two group O secretors and three group A secretors all formed insoluble complexes with concanavalin A. 3. Fractions showing maximum interaction with concanavalin A had maximum blood-group activity measured by the haemagglutination-inhibition technique. The sulphate content of the gastric glycoproteins was unrelated to the capacity to interact with concanavalin A. 4. No interaction was found between concanavalin A and the glycoprotein fractions from any of the saliva or ovarian-cyst-fluid samples tested, implying that there is a structural difference in blood-group-substance glycoproteins in gastric juice when compared with those in saliva and ovarian-cyst fluid. 5. The protein components of each of the secretions tested, gastric juice, saliva and ovarian-cyst fluid, interacted with concanavalin A.
摘要
- 通过在氯化铯密度梯度中进行平衡离心，将胃液、唾液和卵巢囊肿液分离成糖蛋白组分。2. 来自两名O型非分泌者、两名O型分泌者和三名A型分泌者的胃液中的糖蛋白组分均与伴刀豆球蛋白A形成不溶性复合物。3. 通过血凝抑制技术测定，与伴刀豆球蛋白A相互作用最强的组分具有最大的血型活性。胃糖蛋白的硫酸盐含量与与伴刀豆球蛋白A相互作用的能力无关。4. 在测试的任何唾液或卵巢囊肿液样本的糖蛋白组分与伴刀豆球蛋白A之间均未发现相互作用，这意味着与唾液和卵巢囊肿液中的血型物质糖蛋白相比，胃液中的血型物质糖蛋白在结构上存在差异。5. 所测试的每种分泌物（胃液、唾液和卵巢囊肿液）的蛋白质组分均与伴刀豆球蛋白A相互作用。
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