Fagerholm P P, Philipson B T
Invest Ophthalmol Vis Sci. 1979 Nov;18(11):1160-71.
Lens changes caused by injury to the anterior part of the lens were studied with Procion yellow as an extracellular tracer and by transmission electron microscopy at different time intervals after trauma. Both rats and rabbits were used. The findings were related to the slit-lamp appearance of the wounded lenses. In the rat lens a posterior subcapsular cataract developed within the first hour after trauma. Within 1 hr after injury the fluorescent tracer was seen at the wound but was also conspicuous at the posterior pole. Swelling of lens fiber cells and the formation of large syncytical aggregates were found as the posterior opacity enlarged. These changes reached the anterior subcapsular cortex via the equatorial cortex after about 1 month. In the rabbit lens a slight cellular swelling was seen in the subcapsular cortex. Only in one of 15 lenses a posterior subcapsular opacity developed after about 1 week in spite of a large wound. The uptake of Procion yellow was most prominent in the wound area and was never observed at the posterior pole. In both species, no further penetration of the dye occurred through the wound after the epithelium, by regeneration, had sealed the wound. The importance of epithelial wound sealing and that of a restored cellular barrier at the posterior pole are discussed as well as the significance of these factors in the cataract progression.
以普施安黄作为细胞外示踪剂，并通过透射电子显微镜，在创伤后的不同时间间隔，对晶状体前部损伤引起的晶状体变化进行了研究。实验使用了大鼠和兔子。研究结果与受伤晶状体的裂隙灯外观相关。在大鼠晶状体中，创伤后1小时内就出现了后囊下白内障。受伤后1小时内，荧光示踪剂在伤口处可见，但在后极也很明显。随着后极混浊扩大，发现晶状体纤维细胞肿胀并形成大的融合聚集体。大约1个月后，这些变化通过赤道皮质到达前囊下皮质。在兔晶状体中，囊下皮质可见轻微的细胞肿胀。尽管伤口很大，但在15只晶状体中只有1只在大约1周后出现了后囊下混浊。普施安黄的摄取在伤口区域最为明显，在后极从未观察到。在两个物种中，上皮再生封闭伤口后，染料不再通过伤口进一步渗透。本文讨论了上皮伤口封闭的重要性以及后极恢复的细胞屏障的重要性，以及这些因素在白内障进展中的意义。
