Bassel J, Warfel J, Mortimer R
J Bacteriol. 1971 Oct;108(1):609-11. doi: 10.1128/jb.108.1.609-611.1971.
Nutritional requirements were introduced into wild-type, heterothallic strains of Candida lipolytica by exposing the cells to X rays. Complementing hybrids were recovered from mixtures of the auxotrophic strains, and genetic recombination was observed in individually isolated ascospores from the hybrid strains.
通过将解脂耶氏酵母的野生型异宗配合菌株暴露于X射线下,将营养需求引入其中。从营养缺陷型菌株的混合物中获得了互补杂种,并在从杂种菌株中单独分离的子囊中观察到了基因重组。